Linked Life Data

15099759

Source:http://linkedlifedata.com/resource/pubmed/id/15099759

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1-2
pubmed:dateCreated
2004-4-21
pubmed:abstractText
Phagocyte recognition of cells undergoing apoptosis is a rapid, efficient way of removing unwanted cells from tissue. The uptake of apoptotic cells prevents the release of potentially toxic cell contents that might otherwise damage neighbouring cells and elicit an inflammatory response. The aim of this work was to evaluate a simple cell culture assay to study phagocytosis of cells undergoing apoptosis. Fluorescent negatively charged beads (1 microm) or fluorescently labelled apoptotic cells, derived from etoposide-treated human monocytes (U937), were co-incubated with J774 cells or human peripheral blood macrophages for 1 h. Flow cytometry (FCM) showed an efficient uptake of both beads and apoptotic bodies. Phagocytosis of apoptotic cells but not of beads was significantly inhibited when macrophages were pre-incubated with cytochalasin D, suggesting that an experimental system based on beads is not an appropriate model of phagocytosis of apoptotic cells.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0022-1759
pubmed:author
pubmed:issnType
Print
pubmed:volume
287
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
101-8
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
2004
pubmed:articleTitle
Flow cytometric evaluation of a model for phagocytosis of cells undergoing apoptosis.
pubmed:affiliation
Division of Pharmacology, University of Antwerp, Universiteitsplein 1, Wilrijk B-2610, Belgium. dorien.shrijvers@ua.ac.be
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't