Linked Life Data

15095277

Source:http://linkedlifedata.com/resource/pubmed/id/15095277

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
2004-4-19
pubmed:abstractText
Primary Sjögren's syndrome (pSS) is an autoimmune disorder characterized by lymphocytic infiltration of the salivary glands. Most of the infiltrating cells are T cells, but other features of the disease include polyclonal B-cell activation, systemic production of autoantibodies, and increased risk of developing B-cell non-Hodgkin's lymphoma. Recently, a new tumour necrosis factor, the B-cell activating factor (BAFF; also known as BLyS), has been implicated in the polyclonal activation of B cells. Using immunohistochemistry, this study evaluated BAFF expression in labial salivary gland biopsies from 14 patients with pSS, 14 normal controls, and two patients with sarcoidosis. Labial salivary gland samples from seven patients with pSS, seven controls, and one patient with sarcoidosis were double-stained using indirect immunofluorescence. RT-PCR analysis was also performed on lip biopsy samples from two patients and two controls. In all 14 pSS specimens, infiltrating inflammatory cells strongly expressed BAFF protein, as did some ductal epithelial cells, but acinar cells were negative. Some B cells were present in the vicinity of the BAFF-positive cells. In the 14 normal labial salivary glands, some ductal cells were moderately positive, but acinar cells were negative. In the labial salivary glands from the two patients with sarcoidosis, infiltrating lymphocytes were not stained. BAFF mRNA expression was confirmed by RT-PCR in salivary glands from pSS patients. Double immunofluorescence revealed T cells and macrophages to be the main cell types expressing BAFF in salivary glands from pSS patients. In conclusion, BAFF may be expressed by T cells at the site of autoimmune damage and could play a role in the pathogenesis of pSS, particularly by triggering the activation of self-antigen-driven autoimmune B cells.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0022-3417
pubmed:author
pubmed:copyrightInfo
Copyright 2004 Pathological Society of Great Britain and Ireland. Published by John Wiley & Sons, Ltd.
pubmed:issnType
Print
pubmed:volume
202
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
496-502
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:15095277-Adult, pubmed-meshheading:15095277-Aged, pubmed-meshheading:15095277-Antigens, CD3, pubmed-meshheading:15095277-Autoimmunity, pubmed-meshheading:15095277-B-Cell Activating Factor, pubmed-meshheading:15095277-CD4-Positive T-Lymphocytes, pubmed-meshheading:15095277-CD8-Positive T-Lymphocytes, pubmed-meshheading:15095277-Gene Expression, pubmed-meshheading:15095277-Humans, pubmed-meshheading:15095277-Immunoenzyme Techniques, pubmed-meshheading:15095277-Lip, pubmed-meshheading:15095277-Membrane Proteins, pubmed-meshheading:15095277-Middle Aged, pubmed-meshheading:15095277-RNA, Messenger, pubmed-meshheading:15095277-Reverse Transcriptase Polymerase Chain Reaction, pubmed-meshheading:15095277-Salivary Glands, Minor, pubmed-meshheading:15095277-Sjogren's Syndrome, pubmed-meshheading:15095277-T-Lymphocyte Subsets, pubmed-meshheading:15095277-Tumor Necrosis Factor-alpha
pubmed:year
2004
pubmed:articleTitle
Expression of BAFF (BLyS) in T cells infiltrating labial salivary glands from patients with Sjögren's syndrome.
pubmed:affiliation
Service de Rhumatologie, Hôpital de Bicêtre, Assistance Publique-Hôpitaux de Paris, Le Kremlin Bicêtre, France.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't