Linked Life Data

15094194

Source:http://linkedlifedata.com/resource/pubmed/id/15094194

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
2004-4-19
pubmed:databankReference
pubmed:abstractText
The gonadotropin-regulated testicular RNA helicase (GRTH/DDX25) is a new member of the DEAD-box protein family. Phylogenetic analysis revealed that GRTH is distantly related to other members of the family. GRTH is transcriptionally up-regulated by gonadotropin, displays ATPase and RNA helicase activities, and participates in germ cell development. To understand the regulation of GRTH gene expression, we investigated its structural organization and aspects of basal transcriptional regulation at the promoter domain. The 20-kb mouse GRTH gene contains 12 coding exons and all but one of its conserved helicase motifs are contained within single exons. GRTH is a TATA-less gene with multiple transcriptional start sites (TSS), GC-rich sequences and a promoter located within -205/+63 bp of the gene. Sequences -852/-354 and -501/-354 bp caused 40-60% and >80% inhibition of transcription in expressing and non-expressing cells, respectively. Transcriptional activity was recovered only in expressing cells by the addition of upstream sequences (-1085/-852 bp). Sp1/Sp3 supported basal transcriptional activity in all cell types, while E-box was an activator-binding site only in non-expressing cells. These findings indicate that a differential pattern of transcriptional regulation may be involved in the control of GRTH gene expression in a cell-specific manner.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0378-1119
pubmed:author
pubmed:issnType
Print
pubmed:day
28
pubmed:volume
331
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
83-94
pubmed:dateRevised
2009-11-19
pubmed:meshHeading
pubmed-meshheading:15094194-5' Flanking Region, pubmed-meshheading:15094194-Animals, pubmed-meshheading:15094194-Base Sequence, pubmed-meshheading:15094194-Binding Sites, pubmed-meshheading:15094194-Cell Line, pubmed-meshheading:15094194-Cell Line, Tumor, pubmed-meshheading:15094194-DEAD-box RNA Helicases, pubmed-meshheading:15094194-DNA, pubmed-meshheading:15094194-Electrophoretic Mobility Shift Assay, pubmed-meshheading:15094194-Exons, pubmed-meshheading:15094194-Genes, pubmed-meshheading:15094194-Introns, pubmed-meshheading:15094194-Luciferases, pubmed-meshheading:15094194-Mice, pubmed-meshheading:15094194-Mice, Inbred Strains, pubmed-meshheading:15094194-Molecular Sequence Data, pubmed-meshheading:15094194-Nuclear Proteins, pubmed-meshheading:15094194-Phylogeny, pubmed-meshheading:15094194-Promoter Regions, Genetic, pubmed-meshheading:15094194-RNA Helicases, pubmed-meshheading:15094194-Recombinant Fusion Proteins, pubmed-meshheading:15094194-Regulatory Sequences, Nucleic Acid, pubmed-meshheading:15094194-Sequence Analysis, DNA, pubmed-meshheading:15094194-Transcription, Genetic, pubmed-meshheading:15094194-Transcription Initiation Site
pubmed:year
2004
pubmed:articleTitle
Genomic organization and transcriptional analysis of gonadotropin-regulated testicular RNA helicase--GRTH/DDX25 gene.
pubmed:affiliation
Section on Molecular Endocrinology, ERRB, National Institute of Child Health and Human Development, National Institutes of Health, Building 49-6A36, 49 Convent Dr., Bethesda, MD 20892-4510, USA. morrisch@mail.nih.gov
pubmed:publicationType
Journal Article, Comparative Study