Linked Life Data

15084741

Source:http://linkedlifedata.com/resource/pubmed/id/15084741

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
17
pubmed:dateCreated
2004-5-4
pubmed:abstractText
Scopolamine is a pharmaceutically important tropane alkaloid extensively used as an anticholinergic agent. Here, we report the simultaneous introduction and overexpression of genes encoding the rate-limiting upstream enzyme putrescine N-methyltransferase (PMT) and the downstream enzyme hyoscyamine 6 beta-hydroxylase (H6H) of scopolamine biosynthesis in transgenic henbane (Hyoscyamus niger) hairy root cultures. Transgenic hairy root lines expressing both pmt and h6h produced significantly higher (P < 0.05) levels of scopolamine compared with the wild-type and transgenic lines harboring a single gene (pmt or h6h). The best line (T(3)) produced 411 mg/liter scopolamine, which was over nine times more than that in the wild type (43 mg/liter) and more than twice the amount in the highest scopolamine-producing h6h single-gene transgenic line H(11) (184 mg/liter). To our knowledge, this is the highest scopolamine content achieved through genetic engineering of a plant. We conclude that transgenic plants harboring both pmt and h6h possessed an increased flux in the tropane alkaloid biosynthetic pathway that enhanced scopolamine yield, which was more efficient than plants harboring only one of the two genes. It seems that the pulling force of the downstream enzyme (the faucet enzyme) H6H plays a more important role in stimulating scopolamine accumulation in H. niger whereas the functioning of the upstream enzyme PMT is increased proportionally. This study provides an effective approach for large-scale commercial production of scopolamine by using hairy root culture systems as bioreactors.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/15084741-10634784, http://linkedlifedata.com/resource/pubmed/commentcorrection/15084741-11134522, http://linkedlifedata.com/resource/pubmed/commentcorrection/15084741-11909625, http://linkedlifedata.com/resource/pubmed/commentcorrection/15084741-16653064, http://linkedlifedata.com/resource/pubmed/commentcorrection/15084741-16653065, http://linkedlifedata.com/resource/pubmed/commentcorrection/15084741-16664866, http://linkedlifedata.com/resource/pubmed/commentcorrection/15084741-1859872, http://linkedlifedata.com/resource/pubmed/commentcorrection/15084741-3569262, http://linkedlifedata.com/resource/pubmed/commentcorrection/15084741-5650857, http://linkedlifedata.com/resource/pubmed/commentcorrection/15084741-8038607, http://linkedlifedata.com/resource/pubmed/commentcorrection/15084741-9862494
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0027-8424
pubmed:author
pubmed:issnType
Print
pubmed:day
27
pubmed:volume
101
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
6786-91
pubmed:dateRevised
2010-9-21
pubmed:meshHeading
pubmed:year
2004
pubmed:articleTitle
Engineering tropane biosynthetic pathway in Hyoscyamus niger hairy root cultures.
pubmed:affiliation
State Key Laboratory of Genetic Engineering, Morgan-Tan International Center for Life Sciences, School of Life Sciences, Fudan University, Shanghai 200433, China.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't