Switch to
Predicate | Object |
---|---|
rdf:type | |
lifeskim:mentions | |
pubmed:issue |
3
|
pubmed:dateCreated |
1992-9-22
|
pubmed:abstractText |
The c-fos gene expression is rapidly induced by various mitogenic agents and protein synthesis inhibitors in many cell types. Estradiol-17 beta can induce c-fos gene expression in breast cancer cell lines and in the uterus in vivo, but not in cultured guinea-pig endometrial cells. Using this model, we investigated whether a protein synthesis inhibitor, cycloheximide, could induce the c-fos gene and permit a superinduction by estrogens. In the presence of cycloheximide (10 micrograms/ml), protein synthesis was inhibited at 95% within the first hour. From 190 min after the addition of estradiol-17 beta or diethylstilbestrol (10(-8) M) and cycloheximide (10 micrograms/ml), there was a significant increase (ranging from 3- to 5-fold) of the c-fos messenger RNA level (2.2 kilobase in size), compared with the level in cells treated with cycloheximide alone. Nonestrogenic steroid hormones and estradiol-17 alpha were unable to induce c-fos gene expression in the presence of cycloheximide. The effect of estradiol-17 beta observed in the presence of cycloheximide was completely abolished by 4-hydroxy-tamoxifen or by Ly 156758 or by ICI 164384 (10(-6) M). The c-fos mRNAs were rather stable in cells treated with cycloheximide for 2 h (half-life = 51 +/- 6 min) and there was no further increase in the c-fos messenger RNA stability after the addition of cycloheximide plus estradiol-17 beta (half-life = 40 +/- 3 min). The overall results suggest a response at the transcriptional level. In conclusion, cycloheximide transmits activating signals to the c-fos gene which act as priming elements to allow the estrogen action in cultured guinea-pig endometrial cells.
|
pubmed:language |
eng
|
pubmed:journal | |
pubmed:citationSubset |
AIM
|
pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Androstenedione,
http://linkedlifedata.com/resource/pubmed/chemical/Cycloheximide,
http://linkedlifedata.com/resource/pubmed/chemical/Diethylstilbestrol,
http://linkedlifedata.com/resource/pubmed/chemical/Estradiol,
http://linkedlifedata.com/resource/pubmed/chemical/Estriol,
http://linkedlifedata.com/resource/pubmed/chemical/Estrone,
http://linkedlifedata.com/resource/pubmed/chemical/Hydrocortisone,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Tamoxifen,
http://linkedlifedata.com/resource/pubmed/chemical/Testosterone
|
pubmed:status |
MEDLINE
|
pubmed:month |
Sep
|
pubmed:issn |
0013-7227
|
pubmed:author | |
pubmed:issnType |
Print
|
pubmed:volume |
131
|
pubmed:geneSymbol |
c-fos
|
pubmed:owner |
NLM
|
pubmed:authorsComplete |
Y
|
pubmed:pagination |
1094-100
|
pubmed:dateRevised |
2006-11-15
|
pubmed:meshHeading |
pubmed-meshheading:1505453-Androstenedione,
pubmed-meshheading:1505453-Animals,
pubmed-meshheading:1505453-Cells, Cultured,
pubmed-meshheading:1505453-Cycloheximide,
pubmed-meshheading:1505453-Diethylstilbestrol,
pubmed-meshheading:1505453-Endometrium,
pubmed-meshheading:1505453-Estradiol,
pubmed-meshheading:1505453-Estriol,
pubmed-meshheading:1505453-Estrone,
pubmed-meshheading:1505453-Female,
pubmed-meshheading:1505453-Gene Expression,
pubmed-meshheading:1505453-Genes, fos,
pubmed-meshheading:1505453-Guinea Pigs,
pubmed-meshheading:1505453-Hydrocortisone,
pubmed-meshheading:1505453-Kinetics,
pubmed-meshheading:1505453-RNA, Messenger,
pubmed-meshheading:1505453-Tamoxifen,
pubmed-meshheading:1505453-Testosterone,
pubmed-meshheading:1505453-Transcription, Genetic
|
pubmed:year |
1992
|
pubmed:articleTitle |
Superinduction of c-fos gene expression by estrogen in cultured guinea-pig endometrial cells requires priming by a cycloheximide-dependent mechanism.
|
pubmed:affiliation |
INSERM U 198, Besançon, France.
|
pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|