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rdf:type |
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lifeskim:mentions |
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pubmed:dateCreated |
2004-3-31
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pubmed:abstractText |
Novel microreactors with immobilized enzymes were fabricated using both silicon and polymer-based microfabrication techniques. The effectiveness of these reactors was examined along with their behavior over time. Urease enzyme was successfully incorporated into microchannels of a polymeric matrix of polydimethylsiloxane and through layer-bylayer self-assembly techniques onto silicon. The fabricated microchannels had cross-sectional dimensions ranging from tens to hundreds of micrometers in width and height. The experimental results for continuous-flow microreactors are reported for the conversion of urea to ammonia by urease enzyme. Urea conversions of >90% were observed.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:issn |
0273-2289
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
113-116
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
261-72
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:15054211-Biotechnology,
pubmed-meshheading:15054211-Dimethylpolysiloxanes,
pubmed-meshheading:15054211-Enzymes, Immobilized,
pubmed-meshheading:15054211-Fermentation,
pubmed-meshheading:15054211-Microscopy, Electron, Scanning,
pubmed-meshheading:15054211-Nylons,
pubmed-meshheading:15054211-Silicon,
pubmed-meshheading:15054211-Silicones,
pubmed-meshheading:15054211-Time Factors,
pubmed-meshheading:15054211-Urea,
pubmed-meshheading:15054211-Urease
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pubmed:year |
2004
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pubmed:articleTitle |
Immobilized enzyme studies in a microscale bioreactor.
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pubmed:affiliation |
Chemical and Environmental Engineering, The University of Tennessee at Chattanooga, 615 MacCallie Avenue, Chattanooga, TN 37403-2598, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, Non-P.H.S.,
Research Support, Non-U.S. Gov't
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