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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
14
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pubmed:dateCreated |
2004-4-7
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pubmed:abstractText |
Chromosome 3p21.3 region is frequently (>90%) deleted in lung and other major human carcinomas. We subdivided 3p21.3 into LUCA and AP20 subregions and discovered frequent homozygous deletions (10-18%) in both subregions. This finding strongly implies that they harbor multiple tumor suppressor genes involved in the origin and/or development of major epithelial cancers. In this study, we performed an initial analysis of RBSP3/HYA22, a candidate tumor suppressor genes located in the AP20 region. Two sequence splice variants of RBSP3/HYA22 (A and B) were identified, and we provide evidence for their tumor suppressor function. By sequence analysis RBSP3/HYA22 belongs to a gene family of small C-terminal domain phosphatases that may control the RNA polymerase II transcription machinery. Expression of the gene was drastically (>20-fold) decreased in 11 of 12 analyzed carcinoma cell lines and in three of eight tumor biopsies. We report missense and nonsense mutations in tumors where RBSP3/HYA22 was expressed, growth suppression with regulated transgenes in culture, suppression of tumor formation in severe combined immunodeficient mice, and dephosphorylation of ppRB by RBSP3/HYA22, presumably leading to a block of the cell cycle at the G1/S boundary.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/15051889-10371207,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15051889-10888881,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15051889-11085536,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15051889-11161273,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15051889-11390984,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15051889-11477376,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15051889-11826285,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15051889-12024041,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15051889-12124982,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15051889-12149436,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15051889-12362274,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15051889-12543795,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15051889-12721286,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15051889-12771950,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15051889-14566849,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15051889-7959725,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15051889-8485583,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15051889-8603390,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15051889-8634085,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15051889-9111863,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15051889-9179494
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
0027-8424
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pubmed:author |
pubmed-author:BragaEleonoraE,
pubmed-author:ErnbergIngemarI,
pubmed-author:KadyrovaElenaE,
pubmed-author:KashubaVladimir IVI,
pubmed-author:KisselevLev LLL,
pubmed-author:KleinGeorgeG,
pubmed-author:KutsenkoAlexey SAS,
pubmed-author:KuzminIgorI,
pubmed-author:LermanMichael IMI,
pubmed-author:LiJingfengJ,
pubmed-author:MalyukovaAlenaA,
pubmed-author:MinnaJohn DJD,
pubmed-author:MuravenkoOlga VOV,
pubmed-author:ProtopopovAlexeyA,
pubmed-author:SenchenkoVera NVN,
pubmed-author:WangFuliF,
pubmed-author:WinbergGöstaG,
pubmed-author:ZabarovskaVeronika IVI,
pubmed-author:ZabarovskyEugene RER,
pubmed-author:ZeleninAlexander VAV
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pubmed:issnType |
Print
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pubmed:day |
6
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pubmed:volume |
101
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
4906-11
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:15051889-Amino Acid Sequence,
pubmed-meshheading:15051889-Base Sequence,
pubmed-meshheading:15051889-Cell Division,
pubmed-meshheading:15051889-Cell Line, Tumor,
pubmed-meshheading:15051889-DNA Methylation,
pubmed-meshheading:15051889-DNA Primers,
pubmed-meshheading:15051889-DNA Probes,
pubmed-meshheading:15051889-Gene Deletion,
pubmed-meshheading:15051889-Genes, Tumor Suppressor,
pubmed-meshheading:15051889-Humans,
pubmed-meshheading:15051889-Microsatellite Repeats,
pubmed-meshheading:15051889-Molecular Sequence Data,
pubmed-meshheading:15051889-Phosphorylation,
pubmed-meshheading:15051889-Polymerase Chain Reaction,
pubmed-meshheading:15051889-RNA Splicing,
pubmed-meshheading:15051889-Sequence Homology, Amino Acid,
pubmed-meshheading:15051889-Sequence Tagged Sites,
pubmed-meshheading:15051889-Tumor Suppressor Proteins
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pubmed:year |
2004
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pubmed:articleTitle |
RBSP3 (HYA22) is a tumor suppressor gene implicated in major epithelial malignancies.
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pubmed:affiliation |
Microbiology and Tumor Biology Center and Center for Genomics and Bioinformatics, Karolinska Institute, 17177 Stockholm, Sweden.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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