Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
2004-3-29
pubmed:abstractText
Endogenous neural stem/progenitor cells (NSPCs) have recently been shown to differentiate exclusively into astrocytes, the cells that are involved in glial scar formation after spinal cord injury (SCI). The microenvironment of the spinal cord, especially the inflammatory cytokines that dramatically increase in the acute phase at the injury site, is considered to be an important cause of inhibitory mechanism of neuronal differentiation following SCI. Interleukin-6 (IL-6), which has been demonstrated to induce NSPCs to undergo astrocytic differentiation selectively through the JAK/STAT pathway in vitro, has also been demonstrated to play a critical role as a proinflammatory cytokine and to be associated with secondary tissue damage in SCI. In this study, we assessed the efficacy of rat anti-mouse IL-6 receptor monoclonal antibody (MR16-1) in the treatment of acute SCI in mice. Immediately after contusive SCI with a modified NYU impactor, mice were intraperitoneally injected with a single dose of MR16-1 (100 microg/g body weight), the lesions were assessed histologically, and the functional recovery was evaluated. MR16-1 not only suppressed the astrocytic diffentiation-promoting effect of IL-6 signaling in vitro but inhibited the development of astrogliosis after SCI in vivo. MR16-1 also decreased the number of invading inflammatory cells and the severity of connective tissue scar formation. In addition, we observed significant functional recovery in the mice treated with MR16-1 compared with control mice. These findings suggest that neutralization of IL-6 signaling in the acute phase of SCI represents an attractive option for the treatment of SCI.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
http://linkedlifedata.com/resource/pubmed/chemical/Antibodies, http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD11b, http://linkedlifedata.com/resource/pubmed/chemical/Bromodeoxyuridine, http://linkedlifedata.com/resource/pubmed/chemical/DNA-Binding Proteins, http://linkedlifedata.com/resource/pubmed/chemical/Glial Fibrillary Acidic Protein, http://linkedlifedata.com/resource/pubmed/chemical/Hu Paraneoplastic..., http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-6, http://linkedlifedata.com/resource/pubmed/chemical/Nerve Tissue Proteins, http://linkedlifedata.com/resource/pubmed/chemical/RNA-Binding Proteins, http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Interleukin-6, http://linkedlifedata.com/resource/pubmed/chemical/STAT3 Transcription Factor, http://linkedlifedata.com/resource/pubmed/chemical/Stat3 protein, mouse, http://linkedlifedata.com/resource/pubmed/chemical/Trans-Activators
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0360-4012
pubmed:author
pubmed:copyrightInfo
Copyright 2004 Wiley-Liss, Inc.
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
76
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
265-76
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:15048924-Analysis of Variance, pubmed-meshheading:15048924-Animals, pubmed-meshheading:15048924-Antibodies, pubmed-meshheading:15048924-Antigens, CD11b, pubmed-meshheading:15048924-Astrocytes, pubmed-meshheading:15048924-Behavior, Animal, pubmed-meshheading:15048924-Blotting, Western, pubmed-meshheading:15048924-Bromodeoxyuridine, pubmed-meshheading:15048924-Cell Count, pubmed-meshheading:15048924-Cells, Cultured, pubmed-meshheading:15048924-DNA-Binding Proteins, pubmed-meshheading:15048924-Disease Models, Animal, pubmed-meshheading:15048924-Female, pubmed-meshheading:15048924-Glial Fibrillary Acidic Protein, pubmed-meshheading:15048924-Hu Paraneoplastic Encephalomyelitis Antigens, pubmed-meshheading:15048924-Immunohistochemistry, pubmed-meshheading:15048924-Interleukin-6, pubmed-meshheading:15048924-Locomotion, pubmed-meshheading:15048924-Male, pubmed-meshheading:15048924-Mice, pubmed-meshheading:15048924-Mice, Inbred C57BL, pubmed-meshheading:15048924-Nerve Tissue Proteins, pubmed-meshheading:15048924-Psychomotor Performance, pubmed-meshheading:15048924-RNA-Binding Proteins, pubmed-meshheading:15048924-Receptors, Interleukin-6, pubmed-meshheading:15048924-Recovery of Function, pubmed-meshheading:15048924-STAT3 Transcription Factor, pubmed-meshheading:15048924-Spinal Cord Injuries, pubmed-meshheading:15048924-Staining and Labeling, pubmed-meshheading:15048924-Trans-Activators
pubmed:year
2004
pubmed:articleTitle
Blockade of interleukin-6 receptor suppresses reactive astrogliosis and ameliorates functional recovery in experimental spinal cord injury.
pubmed:affiliation
Department of Physiology, Keio University School of Medicine, Shinjuku, Tokyo, Japan.
pubmed:publicationType
Journal Article, Comparative Study, In Vitro, Research Support, Non-U.S. Gov't