Linked Life Data

15048921

Source:http://linkedlifedata.com/resource/pubmed/id/15048921

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
2004-3-29
pubmed:abstractText
Nuclear magnetic resonance (MR) imaging provides a noninvasive method for studying the fate of transplanted cells in vivo. We studied, in animals with a cortical photochemical lesion or with a balloon-induced spinal cord compression lesion, the fate of implanted rat bone marrow stromal cells (MSCs) and mouse embryonic stem cells (ESCs) labeled with superparamagnetic iron oxide nanoparticles (Endorem). MSCs were colabeled with bromodeoxyuridine (BrdU), and ESCs were transfected with pEGFP-C1 (eGFP ESCs). Cells were either grafted intracerebrally into the contralateral hemisphere of the adult rat brain or injected intravenously. In vivo MR imaging was used to track their fate; Prussian blue staining and electron microscopy confirmed the presence of iron oxide nanoparticles inside the cells. During the first week postimplantation, grafted cells migrated to the lesion site and populated the border zone of the lesion. Less than 3% of MSCs differentiated into neurons and none into astrocytes; 5% of eGFP ESCs differentiated into neurons, whereas 70% of eGFP ESCs became astrocytes. The implanted cells were visible on MR images as a hypointense area at the injection site, in the corpus callosum and in the lesion. The hypointense signal persisted for more than 50 days. The presence of GFP-positive or BrdU-positive and nanoparticle-labeled cells was confirmed by histological staining. Our study demonstrates that both grafted MSCs and eGFP ESCs labeled with a contrast agent based on iron oxide nanoparticles migrate into the injured CNS. Iron oxide nanoparticles can therefore be used as a marker for the long-term noninvasive MR tracking of implanted stem cells.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0360-4012
pubmed:author
pubmed:copyrightInfo
Copyright 2004 Wiley-Liss, Inc.
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
76
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
232-43
pubmed:dateRevised
2007-11-15
pubmed:meshHeading
pubmed-meshheading:15048921-Animals, pubmed-meshheading:15048921-Bone Marrow Cells, pubmed-meshheading:15048921-Bone Marrow Transplantation, pubmed-meshheading:15048921-Brain, pubmed-meshheading:15048921-Brain Injuries, pubmed-meshheading:15048921-Cells, Cultured, pubmed-meshheading:15048921-Embryo, Mammalian, pubmed-meshheading:15048921-Ferric Compounds, pubmed-meshheading:15048921-Glial Fibrillary Acidic Protein, pubmed-meshheading:15048921-Green Fluorescent Proteins, pubmed-meshheading:15048921-Immunohistochemistry, pubmed-meshheading:15048921-Luminescent Proteins, pubmed-meshheading:15048921-Magnetic Resonance Imaging, pubmed-meshheading:15048921-Mice, pubmed-meshheading:15048921-Microscopy, Electron, pubmed-meshheading:15048921-Neuroglia, pubmed-meshheading:15048921-Neurons, pubmed-meshheading:15048921-Phosphopyruvate Hydratase, pubmed-meshheading:15048921-Rats, pubmed-meshheading:15048921-Rats, Wistar, pubmed-meshheading:15048921-Spinal Cord, pubmed-meshheading:15048921-Spinal Cord Compression, pubmed-meshheading:15048921-Stem Cell Transplantation, pubmed-meshheading:15048921-Stem Cells
pubmed:year
2004
pubmed:articleTitle
Magnetic resonance tracking of transplanted bone marrow and embryonic stem cells labeled by iron oxide nanoparticles in rat brain and spinal cord.
pubmed:affiliation
Institute of Experimental Medicine Academy of Sciences of the Czech Republic, Prague, Czech Republic.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, Non-U.S. Gov't