| pubmed-article:15048813 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:15048813 | lifeskim:mentions | umls-concept:C0335038 | lld:lifeskim |
| pubmed-article:15048813 | lifeskim:mentions | umls-concept:C0017262 | lld:lifeskim |
| pubmed-article:15048813 | lifeskim:mentions | umls-concept:C0002085 | lld:lifeskim |
| pubmed-article:15048813 | lifeskim:mentions | umls-concept:C0450240 | lld:lifeskim |
| pubmed-article:15048813 | lifeskim:mentions | umls-concept:C1706210 | lld:lifeskim |
| pubmed-article:15048813 | lifeskim:mentions | umls-concept:C2347567 | lld:lifeskim |
| pubmed-article:15048813 | lifeskim:mentions | umls-concept:C2911684 | lld:lifeskim |
| pubmed-article:15048813 | lifeskim:mentions | umls-concept:C0185117 | lld:lifeskim |
| pubmed-article:15048813 | pubmed:issue | 3 | lld:pubmed |
| pubmed-article:15048813 | pubmed:dateCreated | 2004-3-29 | lld:pubmed |
| pubmed-article:15048813 | pubmed:abstractText | Alteration of the mouse genome through homologous recombination in embryonic stem (ES) cells is the most accurate and versatile way to dissect gene function in a vertebrate model. Most often, a selectable marker is used to create a knockout allele by replacing an essential part of the gene. However, knockout strategies are limited because the mutation is present constitutively. Conditional approaches based on the Cre-loxP site-specific recombination (SSR) system address this limitation; however, it requires that all parts of the targeted gene remain in ES cells. Here we report success with a "knockout-first" strategy that ablates gene function by insertion of RNA processing signals without deletion of any of the target gene. Incorporation of site-specific recombination target sites creates a multipurpose allele for both knockout and conditional applications. | lld:pubmed |
| pubmed-article:15048813 | pubmed:language | eng | lld:pubmed |
| pubmed-article:15048813 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15048813 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:15048813 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15048813 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15048813 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15048813 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15048813 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15048813 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15048813 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15048813 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15048813 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15048813 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15048813 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15048813 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15048813 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15048813 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:15048813 | pubmed:month | Mar | lld:pubmed |
| pubmed-article:15048813 | pubmed:issn | 1526-954X | lld:pubmed |
| pubmed-article:15048813 | pubmed:author | pubmed-author:HermannThomas... | lld:pubmed |
| pubmed-article:15048813 | pubmed:author | pubmed-author:StremmelWolfg... | lld:pubmed |
| pubmed-article:15048813 | pubmed:author | pubmed-author:StewartA... | lld:pubmed |
| pubmed-article:15048813 | pubmed:author | pubmed-author:ZhangYoumingY | lld:pubmed |
| pubmed-article:15048813 | pubmed:author | pubmed-author:Anastassiadis... | lld:pubmed |
| pubmed-article:15048813 | pubmed:author | pubmed-author:TestaGiuseppe... | lld:pubmed |
| pubmed-article:15048813 | pubmed:author | pubmed-author:van der... | lld:pubmed |
| pubmed-article:15048813 | pubmed:author | pubmed-author:SchaftJuliaJ | lld:pubmed |
| pubmed-article:15048813 | pubmed:author | pubmed-author:GlaserStefanS | lld:pubmed |
| pubmed-article:15048813 | pubmed:copyrightInfo | Copyright 2004 Wiley-Liss, Inc. | lld:pubmed |
| pubmed-article:15048813 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:15048813 | pubmed:volume | 38 | lld:pubmed |
| pubmed-article:15048813 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:15048813 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:15048813 | pubmed:pagination | 151-8 | lld:pubmed |
| pubmed-article:15048813 | pubmed:dateRevised | 2007-11-15 | lld:pubmed |
| pubmed-article:15048813 | pubmed:meshHeading | pubmed-meshheading:15048813... | lld:pubmed |
| pubmed-article:15048813 | pubmed:meshHeading | pubmed-meshheading:15048813... | lld:pubmed |
| pubmed-article:15048813 | pubmed:meshHeading | pubmed-meshheading:15048813... | lld:pubmed |
| pubmed-article:15048813 | pubmed:meshHeading | pubmed-meshheading:15048813... | lld:pubmed |
| pubmed-article:15048813 | pubmed:meshHeading | pubmed-meshheading:15048813... | lld:pubmed |
| pubmed-article:15048813 | pubmed:meshHeading | pubmed-meshheading:15048813... | lld:pubmed |
| pubmed-article:15048813 | pubmed:meshHeading | pubmed-meshheading:15048813... | lld:pubmed |
| pubmed-article:15048813 | pubmed:meshHeading | pubmed-meshheading:15048813... | lld:pubmed |
| pubmed-article:15048813 | pubmed:meshHeading | pubmed-meshheading:15048813... | lld:pubmed |
| pubmed-article:15048813 | pubmed:meshHeading | pubmed-meshheading:15048813... | lld:pubmed |
| pubmed-article:15048813 | pubmed:meshHeading | pubmed-meshheading:15048813... | lld:pubmed |
| pubmed-article:15048813 | pubmed:meshHeading | pubmed-meshheading:15048813... | lld:pubmed |
| pubmed-article:15048813 | pubmed:meshHeading | pubmed-meshheading:15048813... | lld:pubmed |
| pubmed-article:15048813 | pubmed:meshHeading | pubmed-meshheading:15048813... | lld:pubmed |
| pubmed-article:15048813 | pubmed:meshHeading | pubmed-meshheading:15048813... | lld:pubmed |
| pubmed-article:15048813 | pubmed:meshHeading | pubmed-meshheading:15048813... | lld:pubmed |
| pubmed-article:15048813 | pubmed:meshHeading | pubmed-meshheading:15048813... | lld:pubmed |
| pubmed-article:15048813 | pubmed:meshHeading | pubmed-meshheading:15048813... | lld:pubmed |
| pubmed-article:15048813 | pubmed:meshHeading | pubmed-meshheading:15048813... | lld:pubmed |
| pubmed-article:15048813 | pubmed:meshHeading | pubmed-meshheading:15048813... | lld:pubmed |
| pubmed-article:15048813 | pubmed:year | 2004 | lld:pubmed |
| pubmed-article:15048813 | pubmed:articleTitle | A reliable lacZ expression reporter cassette for multipurpose, knockout-first alleles. | lld:pubmed |
| pubmed-article:15048813 | pubmed:affiliation | Biotec, Technische Universität Dresden, c/o Max Planck Institute of Molecular cell Biology and Genetics, Dresden, Germany. | lld:pubmed |
| pubmed-article:15048813 | pubmed:publicationType | Journal Article | lld:pubmed |
| entrez-gene:17355 | entrezgene:pubmed | pubmed-article:15048813 | lld:entrezgene |
| entrez-gene:26569 | entrezgene:pubmed | pubmed-article:15048813 | lld:entrezgene |
| entrez-gene:56551 | entrezgene:pubmed | pubmed-article:15048813 | lld:entrezgene |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:15048813 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:15048813 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:15048813 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:15048813 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:15048813 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:15048813 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:15048813 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:15048813 | lld:pubmed |
