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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
2004-3-29
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pubmed:abstractText |
Alteration of the mouse genome through homologous recombination in embryonic stem (ES) cells is the most accurate and versatile way to dissect gene function in a vertebrate model. Most often, a selectable marker is used to create a knockout allele by replacing an essential part of the gene. However, knockout strategies are limited because the mutation is present constitutively. Conditional approaches based on the Cre-loxP site-specific recombination (SSR) system address this limitation; however, it requires that all parts of the targeted gene remain in ES cells. Here we report success with a "knockout-first" strategy that ablates gene function by insertion of RNA processing signals without deletion of any of the target gene. Incorporation of site-specific recombination target sites creates a multipurpose allele for both knockout and conditional applications.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Carrier Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Cre recombinase,
http://linkedlifedata.com/resource/pubmed/chemical/DNA-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Fatty Acid Transport Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Integrases,
http://linkedlifedata.com/resource/pubmed/chemical/Membrane Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Membrane Transport Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Mllt2h protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Nuclear Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/RNA,
http://linkedlifedata.com/resource/pubmed/chemical/Slc27a4 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Thioredoxins,
http://linkedlifedata.com/resource/pubmed/chemical/Txn2 protein, mouse
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pubmed:status |
MEDLINE
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pubmed:month |
Mar
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pubmed:issn |
1526-954X
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pubmed:author |
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pubmed:copyrightInfo |
Copyright 2004 Wiley-Liss, Inc.
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pubmed:issnType |
Print
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pubmed:volume |
38
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
151-8
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pubmed:dateRevised |
2007-11-15
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pubmed:meshHeading |
pubmed-meshheading:15048813-Animals,
pubmed-meshheading:15048813-Carrier Proteins,
pubmed-meshheading:15048813-DNA-Binding Proteins,
pubmed-meshheading:15048813-Fatty Acid Transport Proteins,
pubmed-meshheading:15048813-Female,
pubmed-meshheading:15048813-Gene Silencing,
pubmed-meshheading:15048813-Genes, Reporter,
pubmed-meshheading:15048813-Genetic Engineering,
pubmed-meshheading:15048813-Genetic Vectors,
pubmed-meshheading:15048813-Integrases,
pubmed-meshheading:15048813-Lac Operon,
pubmed-meshheading:15048813-Membrane Proteins,
pubmed-meshheading:15048813-Membrane Transport Proteins,
pubmed-meshheading:15048813-Mice,
pubmed-meshheading:15048813-Mice, Knockout,
pubmed-meshheading:15048813-Nuclear Proteins,
pubmed-meshheading:15048813-RNA,
pubmed-meshheading:15048813-Recombination, Genetic,
pubmed-meshheading:15048813-Thioredoxins,
pubmed-meshheading:15048813-Transfection
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pubmed:year |
2004
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pubmed:articleTitle |
A reliable lacZ expression reporter cassette for multipurpose, knockout-first alleles.
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pubmed:affiliation |
Biotec, Technische Universität Dresden, c/o Max Planck Institute of Molecular cell Biology and Genetics, Dresden, Germany.
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pubmed:publicationType |
Journal Article
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