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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
4
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pubmed:dateCreated |
2004-4-2
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pubmed:abstractText |
We describe a transcriptional analysis platform consisting of a universal micro-array system (UMAS) combined with an enzymatic manipulation step that is capable of generating expression profiles from any organism without requiring a priori species-specific knowledge of transcript sequences. The transcriptome is converted to cDNA and processed with restriction endonucleases to generate low-complexity pools (approximately 80-120) of equal length DNA fragments. The resulting material is amplified and detected with the UMAS system, comprising all possible 4,096 (4(6)) DNA hexamers. Ligation to the arrays yields thousands of 14-mer sequence tags. The compendium of signals from all pools in the array-of-universal arrays comprises a full-transcriptome expression profile. The technology was validated by analysis of the galactose response of Saccharomyces cerevisiae, and the resulting profiles showed excellent agreement with the literature and real-time PCR assays. The technology was also used to demonstrate expression profiling from a hybrid organism in a proof-of-concept experiment where a T-cell receptor gene was expressed in yeast.
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pubmed:commentsCorrections |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
1087-0156
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pubmed:author |
pubmed-author:AffourtitJason PJP,
pubmed-author:FordMaura JMJ,
pubmed-author:GrahamCarol DCD,
pubmed-author:GuccioneLisaL,
pubmed-author:GuccioneLorriL,
pubmed-author:GuerraCesar ECE,
pubmed-author:HallingConradC,
pubmed-author:HariharanJayashreeJ,
pubmed-author:HnathJonathanJ,
pubmed-author:IntrieriGinoG,
pubmed-author:JedrzkiewiczBarbaraB,
pubmed-author:KaufmanJoseph CJC,
pubmed-author:KimJunhyongJ,
pubmed-author:KranzDavid MDM,
pubmed-author:KrishnaswamyHarishH,
pubmed-author:LaceyMichelleM,
pubmed-author:LaneJenniferJ,
pubmed-author:LatimerDarin RDR,
pubmed-author:LiFengF,
pubmed-author:LiaoJiJ,
pubmed-author:LizardiPaul MPM,
pubmed-author:MattessichMartin JMJ,
pubmed-author:McConnellKevin JKJ,
pubmed-author:MerkelJane SJS,
pubmed-author:MichaelsonChrisC,
pubmed-author:OrrBrentB,
pubmed-author:ParmanCraig ECE,
pubmed-author:PerbostClotildeC,
pubmed-author:PiperKevin RKR,
pubmed-author:PowellStephen WSW,
pubmed-author:RahaDebasishD,
pubmed-author:RothMatthew EME,
pubmed-author:SchafferPaul JPJ,
pubmed-author:TevelevAntonA,
pubmed-author:ValerioAnthonyA,
pubmed-author:WangRixinR,
pubmed-author:YingBaogeB,
pubmed-author:ZollaBrendaB
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pubmed:issnType |
Print
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pubmed:volume |
22
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
418-26
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:15024387-3' Untranslated Regions,
pubmed-meshheading:15024387-Algorithms,
pubmed-meshheading:15024387-Animals,
pubmed-meshheading:15024387-DNA, Complementary,
pubmed-meshheading:15024387-DNA Fragmentation,
pubmed-meshheading:15024387-DNA Restriction Enzymes,
pubmed-meshheading:15024387-Galactose,
pubmed-meshheading:15024387-Gene Expression Profiling,
pubmed-meshheading:15024387-Humans,
pubmed-meshheading:15024387-Image Processing, Computer-Assisted,
pubmed-meshheading:15024387-Mice,
pubmed-meshheading:15024387-Models, Genetic,
pubmed-meshheading:15024387-Muscle, Skeletal,
pubmed-meshheading:15024387-Muscles,
pubmed-meshheading:15024387-Oligonucleotide Array Sequence Analysis,
pubmed-meshheading:15024387-Promoter Regions, Genetic,
pubmed-meshheading:15024387-RNA, Messenger,
pubmed-meshheading:15024387-Reverse Transcriptase Polymerase Chain Reaction,
pubmed-meshheading:15024387-Saccharomyces cerevisiae,
pubmed-meshheading:15024387-Sequence Analysis, DNA,
pubmed-meshheading:15024387-T-Lymphocytes,
pubmed-meshheading:15024387-Transgenes
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pubmed:year |
2004
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pubmed:articleTitle |
Expression profiling using a hexamer-based universal microarray.
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pubmed:affiliation |
Agilix Corporation, 2 Church Street South, New Haven, Connecticut 06519, USA.
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pubmed:publicationType |
Journal Article
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