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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
7
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pubmed:dateCreated |
2004-3-15
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pubmed:abstractText |
We recently demonstrated that heme oxygenase (HO)-1 is constitutively expressed in human CD4+CD25+ regulatory T cells and induced by anti-CD28 or anti-CD28/anti-CD3 stimulation, even in CD4+CD25- responder T cells. To study the effects of HO-1 expression on lymphocyte survival, we transfected the HO-1 gene or induced the gene to express HO-1 protein with cobalt protoporphyrin (CoPP) in Jurkat T cells. Consistently, anti-Fas antibody triggered apoptotic cell death in wild-type Jurkat T cells. Surprisingly, however, HO-1-overexpressing Jurkat T cells showed strong resistance to Fas-mediated apoptosis. In contrast, abrogation of HO-1 expression by antisense oligomer against HO-1 gene from CoPP-treated cells or depletion of iron by desferrioxamine from HO-1-transfected cells abolished the resistance. In addition, exogenously added iron rendered wild-type Jurkat T cells resistant. The resistance involved IkappaB kinase (IKK) activation via iron-induced reactive oxygen species formation, NF-kappaB activation by activated IKK, and c-FLIP expression by activated NF-kappaB. Primary CD4+ T cells induced by CoPP to express HO-1 also showed more resistance to Fas-mediated apoptosis than untreated cells. Our findings suggest that HO-1 plays a critical and nonredundant role in Fas-mediated activation-induced cell death of T lymphocytes.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD95,
http://linkedlifedata.com/resource/pubmed/chemical/CASP8 and FADD-Like Apoptosis...,
http://linkedlifedata.com/resource/pubmed/chemical/CFLAR protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Caspases,
http://linkedlifedata.com/resource/pubmed/chemical/DNA, Complementary,
http://linkedlifedata.com/resource/pubmed/chemical/HMOX1 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Heme Oxygenase (Decyclizing),
http://linkedlifedata.com/resource/pubmed/chemical/Heme Oxygenase-1,
http://linkedlifedata.com/resource/pubmed/chemical/Intracellular Signaling Peptides...,
http://linkedlifedata.com/resource/pubmed/chemical/Iron,
http://linkedlifedata.com/resource/pubmed/chemical/Membrane Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/NF-kappa B,
http://linkedlifedata.com/resource/pubmed/chemical/NF-kappa B kinase,
http://linkedlifedata.com/resource/pubmed/chemical/Oligonucleotides, Antisense,
http://linkedlifedata.com/resource/pubmed/chemical/Protein-Serine-Threonine Kinases,
http://linkedlifedata.com/resource/pubmed/chemical/Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Protoporphyrins,
http://linkedlifedata.com/resource/pubmed/chemical/Reactive Oxygen Species,
http://linkedlifedata.com/resource/pubmed/chemical/cobaltiprotoporphyrin
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pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
0891-5849
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
1
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pubmed:volume |
36
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
858-71
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pubmed:dateRevised |
2011-11-2
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pubmed:meshHeading |
pubmed-meshheading:15019971-Antigens, CD95,
pubmed-meshheading:15019971-Apoptosis,
pubmed-meshheading:15019971-CASP8 and FADD-Like Apoptosis Regulating Protein,
pubmed-meshheading:15019971-CD4-Positive T-Lymphocytes,
pubmed-meshheading:15019971-Caspases,
pubmed-meshheading:15019971-DNA, Complementary,
pubmed-meshheading:15019971-Flow Cytometry,
pubmed-meshheading:15019971-Genes, bcl-2,
pubmed-meshheading:15019971-Heme Oxygenase (Decyclizing),
pubmed-meshheading:15019971-Heme Oxygenase-1,
pubmed-meshheading:15019971-Humans,
pubmed-meshheading:15019971-Immunochemistry,
pubmed-meshheading:15019971-Intracellular Signaling Peptides and Proteins,
pubmed-meshheading:15019971-Iron,
pubmed-meshheading:15019971-Jurkat Cells,
pubmed-meshheading:15019971-Membrane Proteins,
pubmed-meshheading:15019971-NF-kappa B,
pubmed-meshheading:15019971-Oligonucleotides, Antisense,
pubmed-meshheading:15019971-Protein-Serine-Threonine Kinases,
pubmed-meshheading:15019971-Proteins,
pubmed-meshheading:15019971-Protoporphyrins,
pubmed-meshheading:15019971-Reactive Oxygen Species,
pubmed-meshheading:15019971-Transfection
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pubmed:year |
2004
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pubmed:articleTitle |
Overexpression of heme oxygenase (HO)-1 renders Jurkat T cells resistant to fas-mediated apoptosis: involvement of iron released by HO-1.
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pubmed:affiliation |
Genomic Research Center for Immune Disorders and Department of Microbiology and Immunology, Wonkwang University School of Medicine, Iksan, Chonbuk, Republic of Korea.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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