Source:http://linkedlifedata.com/resource/pubmed/id/15016592
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
3
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pubmed:dateCreated |
2004-3-12
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pubmed:abstractText |
The effects of dithiol chelating agents meso-2,3-dimercaptosuccinic acid (DMSA), 2,3-dimercaptopropane-1-sulfonic acid (DMPS), and 2,3-dimercaptopropanol (BAL) on delta-aminolevulinate dehydratase (delta-ALA-D) from human erythrocytes were evaluated. Furthermore, possible protective effects of zinc chloride (ZnCl(2)), dithiothreitol (DTT), and cysteine were studied. delta-ALA-D activity from human erythrocytes was inhibited by dithiol chelating agents in a concentration-dependent manner. Cysteine, at all concentrations tested, did not protect the inhibitory effect of 1 and 4 mM DMPS and DMSA, but protected 1 mM BAL inhibition. Dithiotreitol was able to protect the inhibition caused by 1 mM BAL (28%), DMPS (56%), and DMSA (40%) in a concentration-dependent manner. Zinc chloride protected and restored 1 mM BAL inhibitory effect on delta-ALA-D. Zinc chloride at 500 microM and 1 mM, respectively, protected inhibitory effects of DMPS and DMSA (1 and 4 mM), but did not reverse its effects. The preincubation of dithiol chelating agents with enzyme demonstrated that DMSA was the most potent delta-ALA-D inhibitor of human erythrocytes. These data are in agreement with delta-ALA-D activity from purified enzyme. ZnCl(2) (1 microM) added, in the reaction mixture, increased enzyme activity and DTT (100 microM) totally restored the enzyme activity for all chelating agents tested.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Chelating Agents,
http://linkedlifedata.com/resource/pubmed/chemical/Chlorides,
http://linkedlifedata.com/resource/pubmed/chemical/Cysteine,
http://linkedlifedata.com/resource/pubmed/chemical/Dimercaprol,
http://linkedlifedata.com/resource/pubmed/chemical/Dithiothreitol,
http://linkedlifedata.com/resource/pubmed/chemical/Porphobilinogen Synthase,
http://linkedlifedata.com/resource/pubmed/chemical/Reducing Agents,
http://linkedlifedata.com/resource/pubmed/chemical/Succimer,
http://linkedlifedata.com/resource/pubmed/chemical/Unithiol,
http://linkedlifedata.com/resource/pubmed/chemical/Zinc Compounds,
http://linkedlifedata.com/resource/pubmed/chemical/zinc chloride
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pubmed:status |
MEDLINE
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pubmed:month |
Mar
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pubmed:issn |
0013-9351
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
94
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
254-61
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:15016592-Chelating Agents,
pubmed-meshheading:15016592-Chlorides,
pubmed-meshheading:15016592-Cysteine,
pubmed-meshheading:15016592-Dimercaprol,
pubmed-meshheading:15016592-Dithiothreitol,
pubmed-meshheading:15016592-Dose-Response Relationship, Drug,
pubmed-meshheading:15016592-Erythrocytes,
pubmed-meshheading:15016592-Humans,
pubmed-meshheading:15016592-Porphobilinogen Synthase,
pubmed-meshheading:15016592-Reducing Agents,
pubmed-meshheading:15016592-Succimer,
pubmed-meshheading:15016592-Unithiol,
pubmed-meshheading:15016592-Zinc Compounds
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pubmed:year |
2004
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pubmed:articleTitle |
2,3-Dimercaptopropanol, 2,3-dimercaptopropane-1-sulfonic acid, and meso-2,3-dimercaptosuccinic acid inhibit delta-aminolevulinate dehydratase from human erythrocytes in vitro.
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pubmed:affiliation |
Departamento de Química, Centro de Ciências Naturais e Exatas, Universidade Federal de Santa Maria, Santa Maria CEP 97105-900, RS, Brazil. criswn@quimica.ufsm.br
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pubmed:publicationType |
Journal Article,
Comparative Study,
In Vitro
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