Linked Life Data

15009651

Source:http://linkedlifedata.com/resource/pubmed/id/15009651

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
2004-3-10
pubmed:abstractText
Regulation of intracellular cyclic AMP is critical to the modulation of many cellular activities, including cellular differentiation. Moreover, morphological differentiation has been linked to subsequent alterations in the cAMP signaling pathway in various cellular models. The current study was designed to explore the mechanism for the previously reported enhancement of adenylate cyclase activity in Cath.a differentiated cells following differentiation. Differentiation of Cath.a differentiated cells stably expressing the D2L dopamine receptor markedly potentiated both forskolin- and A2-adenosine receptor-stimulated cAMP accumulation. This enhancement was accompanied by a twofold increase in adenylate cyclase 6 (AC6) expression and a dramatic loss in the expression of AC9. The ability of Ca2+ to inhibit drug-stimulated cAMP accumulation was enhanced following differentiation, as was D2L dopamine receptor-mediated inhibition of Galphas-stimulated cAMP accumulation. Differentiation altered basal and drug-stimulated phosphorylation of the cAMP-response element-binding protein, which was independent of changes in protein kinase A expression. The current data suggest that differentiation of the neuronal cell model, Cath.a differentiated cells induces significant alterations in the expression and function of both the proximal and distal portions of the cAMP signaling pathway and may impact cellular operations dependent upon this pathway.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
0022-3042
pubmed:author
pubmed:issnType
Print
pubmed:volume
88
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1497-508
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed-meshheading:15009651-Adenylate Cyclase, pubmed-meshheading:15009651-Animals, pubmed-meshheading:15009651-Calcium, pubmed-meshheading:15009651-Cell Differentiation, pubmed-meshheading:15009651-Cell Line, pubmed-meshheading:15009651-Culture Media, Serum-Free, pubmed-meshheading:15009651-Cyclic AMP, pubmed-meshheading:15009651-Cyclic AMP Response Element-Binding Protein, pubmed-meshheading:15009651-Dose-Response Relationship, Drug, pubmed-meshheading:15009651-Forskolin, pubmed-meshheading:15009651-GTP-Binding Proteins, pubmed-meshheading:15009651-Humans, pubmed-meshheading:15009651-Isoenzymes, pubmed-meshheading:15009651-Mice, pubmed-meshheading:15009651-Neurons, pubmed-meshheading:15009651-Phosphorylation, pubmed-meshheading:15009651-Receptors, Adenosine A2, pubmed-meshheading:15009651-Receptors, Dopamine D2, pubmed-meshheading:15009651-Signal Transduction, pubmed-meshheading:15009651-Transfection
pubmed:year
2004
pubmed:articleTitle
Differentiation-induced alterations in cyclic AMP signaling in the Cath.a differentiated (CAD) neuronal cell line.
pubmed:affiliation
Department of Medicinal Chemistry and Molecular Pharmacology, Purdue University, West Lafayette, Indiana 47907, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't