Linked Life Data

15007843

Source:http://linkedlifedata.com/resource/pubmed/id/15007843

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2004-3-9
pubmed:abstractText
Deoxyribozyme (DNAzyme) carrying peroxidase activity was immobilized on two types of particles and the enzymatic activity was measured. The DNA recognizing porphyrin were prepared according to Travascio et al. ([1998] Chem Biol 5:505-517) and the interactions with hemin were investigated by ultraviolet absorbance and circular dichroism spectroscopies. The DNA interacted with hemin and significant conformational change was induced by the interaction. Therefore, the end of this DNA was modified with a thiol group and it was immobilized on thiol-containing polysaccharide beads or on gold particles. The DNA immobilized on the gold particle showed activity catalyzing the peroxidation reaction. No significant reduction of activity was observed even after immobilization. The immobilized DNAzyme could be repeatedly utilized without significant loss of activity. In addition, heat treatment did not reduce the activity, although a protein enzyme, horseradish peroxidase, lost its activity after the heat treatment. The repertoire of DNAzyme is still currently limited. However, in the future the utilization of DNAzyme in the field of biotechnology will be important with the increase of discoveries of new functional DNAzymes.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0006-3592
pubmed:author
pubmed:copyrightInfo
Copyright 2004 Wiley Periodicals, Inc.
pubmed:issnType
Print
pubmed:day
5
pubmed:volume
86
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
72-7
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed:year
2004
pubmed:articleTitle
Immobilization of DNAzyme as a thermostable biocatalyst.
pubmed:affiliation
Kanagawa Academy of Science and Technology, KSP East 309, 3-2-1 Sakado, Takatsu-ku, Kawasaki 213-0012, Japan.
pubmed:publicationType
Journal Article, Comparative Study, Evaluation Studies