Linked Life Data

14993787

Source:http://linkedlifedata.com/resource/pubmed/id/14993787

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
2004-3-2
pubmed:abstractText
The formation of cyclic 1,N2-propano guanine (CPr-Gua) adduct is significantly accelerated by the addition of arginine or histone in the reaction of calf thymus DNA with acetaldehyde (AA) or crotonaldehyde (CA). Histone proteins, containing a large amount of basic amino acids such as arginine, are essential as nucleosome cores to package DNA. In the presence of 0.60% (w/v) histone in the reaction mixture, 8-times and 25-times larger amounts of the CPr-Gua adduct were formed in the reaction of the DNA with AA and CA, respectively, compared with those in the absence of histone. Furthermore, for the DNA incubated at 95 degrees C for 10 min and cooled on ice to make the single-stranded moieties, 72-times and 178-times larger amounts of the CPr-Gua adduct were formed by AA and CA, respectively, in the presence of 0.60% (w/v) histone. These results strongly suggest that DNA in vivo should be exposed to a much more dangerous situation, compared with DNA alone, from the viewpoint of reactivity with the aldehydes. During DNA replication and transcriptional events of cells, the danger will be further increased markedly because of opening of double-strands. Semi-micro HPLC-ESI-MS measurements following deprination of DNA samples were performed for quantification of the adduct as the corresponding base form, CPr-Gua, in evaluation of the reactivity of DNA with AA and CA.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
0918-6158
pubmed:author
pubmed:issnType
Print
pubmed:volume
27
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
273-6
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
2004
pubmed:articleTitle
LC-MS study on the formation of cyclic 1,N2-propano guanine adduct in the reactions of DNA with acetaldehyde in the presence of histone.
pubmed:affiliation
Gifu Pharmaceutical University, Mitahora-higashi, Gifu, Japan.
pubmed:publicationType
Journal Article