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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
1992-9-4
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pubmed:abstractText |
The guanine nucleotide dependence for the generation of inositolglycan second messengers from rat liver plasma membranes has been investigated. Plasma membranes, when treated with insulin release a soluble mediator substance which activates pyruvate dehydrogenase (PDH). Guanosine 5'-[3-thio]triphosphate (GTP gamma S) was found to be as potent as insulin in stimulating mediator release. The stimulatory effects of GTP gamma S required the presence of magnesium and following preincubation of membranes with guanosine 5'-[2-thio]diphosphate (GDP beta S) the stimulation of mediator release by either insulin or GTP gamma S was blocked. The activation of PDH by mediator fractions produced in response to either insulin or GTP gamma S was abolished following treatment of the fractions with anti-inositolglycan antibodies. The significance of these observations with respect to the possible involvement of a regulatory guanine-nucleotide binding protein (G-protein) in the generation of insulin mediators is discussed.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
0006-291X
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
31
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pubmed:volume |
186
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1151-7
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pubmed:dateRevised |
2011-11-17
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pubmed:meshHeading |
pubmed-meshheading:1497649-Adenosine Triphosphate,
pubmed-meshheading:1497649-Animals,
pubmed-meshheading:1497649-Antibodies,
pubmed-meshheading:1497649-Cell Membrane,
pubmed-meshheading:1497649-Dose-Response Relationship, Drug,
pubmed-meshheading:1497649-GTP-Binding Proteins,
pubmed-meshheading:1497649-Guanine Nucleotides,
pubmed-meshheading:1497649-Guanosine 5'-O-(3-Thiotriphosphate),
pubmed-meshheading:1497649-Inositol,
pubmed-meshheading:1497649-Insulin,
pubmed-meshheading:1497649-Kinetics,
pubmed-meshheading:1497649-Liver,
pubmed-meshheading:1497649-Male,
pubmed-meshheading:1497649-Polysaccharides,
pubmed-meshheading:1497649-Pyruvate Dehydrogenase Complex,
pubmed-meshheading:1497649-Rats,
pubmed-meshheading:1497649-Rats, Inbred Strains,
pubmed-meshheading:1497649-Second Messenger Systems
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pubmed:year |
1992
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pubmed:articleTitle |
The generation of inositolglycan mediators from rat liver plasma membranes: the role of guanine nucleotide binding proteins.
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pubmed:affiliation |
Department of Pharmacology, University of Virginia Health Sciences Center, Charlottesville 22908.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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