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rdf:type |
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lifeskim:mentions |
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pubmed:dateCreated |
1992-9-10
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pubmed:abstractText |
A monoclonal IgG-1 was produced by culture of a murine hybridoma (3.8.6) by three different methods, namely culture in ascites, in serum-free media and in serum-supplemented media. IgG-1 was purified to homogeneity (as judged by SDS/PAGE under reducing conditions) from each medium by ion-exchange chromatography and h.p.l.c. Protein A chromatography. Oligosaccharides were released from each IgG-1 preparation by hydrazinolysis and radiolabelled by reduction with alkaline sodium borotritide, and 'profile' analysis of the radiolabelled oligosaccharide alditols was performed by a combination of paper electrophoresis and gel-filtration chromatography. This analysis indicated clear and reproducible differences in the glycosylation patterns of the three IgG-1 preparations. Sequential exoglycosidase analysis of individual oligosaccharides derived from each IgG-1 preparation was used to define these differences. Ascites-derived material differed from serum-free-culture-derived material only with respect to the content of sialic acid. IgG-1 derived from culture in serum-containing media had an intermediate sialic acid content and a lower incidence of outer-arm galactosylation than the other two preparations. These differences in glycosylation could not be induced in any IgG-1 preparation by incubating purified IgG-1 with ascites or culture medium. It is concluded that the glycosylation pattern of a secreted monoclonal IgG is dependent on the culture method employed to obtain it.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/1497622-1366536,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1497622-2125204,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1497622-2504180,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1497622-2886334,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1497622-3102493,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1497622-3275561,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1497622-3457370,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1497622-3537734,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1497622-3621680,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1497622-367955,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1497622-3814165,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1497622-3896128,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1497622-3927174,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1497622-4083905,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1497622-4193390,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1497622-5458650,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1497622-6181710,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1497622-6579549,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1497622-6873448,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1497622-85629
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
0264-6021
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
1
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pubmed:volume |
285 ( Pt 3)
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
839-45
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pubmed:dateRevised |
2010-9-7
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pubmed:meshHeading |
pubmed-meshheading:1497622-Animals,
pubmed-meshheading:1497622-Antibodies, Monoclonal,
pubmed-meshheading:1497622-Ascitic Fluid,
pubmed-meshheading:1497622-Blood,
pubmed-meshheading:1497622-Carbohydrate Sequence,
pubmed-meshheading:1497622-Cells, Cultured,
pubmed-meshheading:1497622-Chromatography, Gel,
pubmed-meshheading:1497622-Culture Media,
pubmed-meshheading:1497622-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:1497622-Glycoside Hydrolases,
pubmed-meshheading:1497622-Glycosylation,
pubmed-meshheading:1497622-Hybridomas,
pubmed-meshheading:1497622-Immunoglobulin G,
pubmed-meshheading:1497622-Mice,
pubmed-meshheading:1497622-Molecular Sequence Data,
pubmed-meshheading:1497622-N-Acetylneuraminic Acid,
pubmed-meshheading:1497622-Oligosaccharides,
pubmed-meshheading:1497622-Sialic Acids
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pubmed:year |
1992
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pubmed:articleTitle |
Different culture methods lead to differences in glycosylation of a murine IgG monoclonal antibody.
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pubmed:affiliation |
Oxford GlycoSystem Ltd., Abingdon, Oxen, U.K.
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pubmed:publicationType |
Journal Article,
Comparative Study
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