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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
2004-2-19
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pubmed:abstractText |
The Epstein-Barr virus nuclear antigen (EBNA)-6 protein is essential for Epstein-Barr virus (EBV)-induced immortalization of primary human B-lymphocytes in vitro. In this study, fusion proteins of EBNA-6 with green fluorescent protein (GFP) have been used to characterize its nuclear localization and organization within the nucleus. EBNA-6 associates with nuclear structures and in immunofluorescence demonstrate a punctate staining pattern. Herein, we show that the association of EBNA-6 with these nuclear structures was maintained throughout the cell cycle and with the use of GFP-E6 deletion mutants, that the region amino acids 733-808 of EBNA-6 contains a domain that can influence the association of EBNA-6 with these nuclear structures. Co-immunofluorescence and confocal analyses demonstrated that EBNA-6 and EBNA-3 co-localize in the nucleus of cells. Expression of EBNA-6, but not EBNA-3, caused a redistribution of nuclear survival of motor neurons protein (SMN) to the EBNA-6 containing nuclear structures resulting in co-localization of SMN with EBNA-6.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jan
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pubmed:issn |
0042-6822
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
5
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pubmed:volume |
318
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
280-94
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:14972554-Cell Line, Tumor,
pubmed-meshheading:14972554-Cell Nucleus,
pubmed-meshheading:14972554-Cell Nucleus Structures,
pubmed-meshheading:14972554-Cell Transformation, Viral,
pubmed-meshheading:14972554-Cyclic AMP Response Element-Binding Protein,
pubmed-meshheading:14972554-Epstein-Barr Virus Nuclear Antigens,
pubmed-meshheading:14972554-Gene Deletion,
pubmed-meshheading:14972554-Green Fluorescent Proteins,
pubmed-meshheading:14972554-HeLa Cells,
pubmed-meshheading:14972554-Herpesvirus 4, Human,
pubmed-meshheading:14972554-Humans,
pubmed-meshheading:14972554-Luminescent Proteins,
pubmed-meshheading:14972554-Nerve Tissue Proteins,
pubmed-meshheading:14972554-RNA-Binding Proteins,
pubmed-meshheading:14972554-Recombinant Fusion Proteins,
pubmed-meshheading:14972554-SMN Complex Proteins
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pubmed:year |
2004
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pubmed:articleTitle |
The Epstein-Barr virus nuclear antigen-6 protein co-localizes with EBNA-3 and survival of motor neurons protein.
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pubmed:affiliation |
Queensland Institute of Medical Research and ACITHN University of Queensland, Brisbane 4029, Queensland, Australia.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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