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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
4
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pubmed:dateCreated |
2004-2-17
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pubmed:abstractText |
To reveal the molecular mechanism of selective follicular atresia in porcine ovaries, we investigated the changes in the expression of tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) and its receptor (DR4) proteins and TRAIL mRNA in granulosa cells during follicular atresia. Immunohistochemical, Western immunoblotting and reverse transcription-polymerase chain reaction analyses (RT-PCR) revealed that significant increases in TRAIL protein and mRNA levels but not DR4 protein were changed during atresia. The RT-PCR product was confirmed to be porcine TRAIL by the cDNA sequence determination. An in vitro apoptosis inducing assay using cultured granulosa cells prepared from healthy follicles showed that TRAIL could activate caspase-3 and induce apoptotic cell death in the cells. The present findings confirm that TRAIL induces apoptosis in granulosa cells during atresia in porcine ovaries.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Apoptosis Regulatory Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/CASP3 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Caspase 3,
http://linkedlifedata.com/resource/pubmed/chemical/Caspases,
http://linkedlifedata.com/resource/pubmed/chemical/DNA, Complementary,
http://linkedlifedata.com/resource/pubmed/chemical/Membrane Glycoproteins,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, TNF-Related...,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Tumor Necrosis Factor,
http://linkedlifedata.com/resource/pubmed/chemical/TNF-Related Apoptosis-Inducing...,
http://linkedlifedata.com/resource/pubmed/chemical/TNFRSF10A protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/TNFSF10 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Tumor Necrosis Factor-alpha
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pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
0916-8818
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
49
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
313-21
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pubmed:dateRevised |
2011-11-17
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pubmed:meshHeading |
pubmed-meshheading:14967924-Animals,
pubmed-meshheading:14967924-Apoptosis,
pubmed-meshheading:14967924-Apoptosis Regulatory Proteins,
pubmed-meshheading:14967924-Base Sequence,
pubmed-meshheading:14967924-Caspase 3,
pubmed-meshheading:14967924-Caspases,
pubmed-meshheading:14967924-DNA, Complementary,
pubmed-meshheading:14967924-Enzyme Activation,
pubmed-meshheading:14967924-Female,
pubmed-meshheading:14967924-Follicular Atresia,
pubmed-meshheading:14967924-Gene Expression,
pubmed-meshheading:14967924-Granulosa Cells,
pubmed-meshheading:14967924-Membrane Glycoproteins,
pubmed-meshheading:14967924-Ovary,
pubmed-meshheading:14967924-RNA, Messenger,
pubmed-meshheading:14967924-Receptors, TNF-Related Apoptosis-Inducing Ligand,
pubmed-meshheading:14967924-Receptors, Tumor Necrosis Factor,
pubmed-meshheading:14967924-Signal Transduction,
pubmed-meshheading:14967924-Sus scrofa,
pubmed-meshheading:14967924-TNF-Related Apoptosis-Inducing Ligand,
pubmed-meshheading:14967924-Tumor Necrosis Factor-alpha
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pubmed:year |
2003
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pubmed:articleTitle |
Roles of tumor necrosis factor-related apoptosis-inducing ligand signaling pathway in granulosa cell apoptosis during atresia in pig ovaries.
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pubmed:affiliation |
Unit of Anatomy and Cell Biology, Department of Animal Sciences, Kyoto University, Kyoto 806-8502, Japan.
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pubmed:publicationType |
Journal Article,
In Vitro,
Research Support, Non-U.S. Gov't
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