Linked Life Data

14961232

Source:http://linkedlifedata.com/resource/pubmed/id/14961232

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
2004-2-12
pubmed:abstractText
The effects of theonellamide F, a marine bicyclic peptide, on vacuolar formation in cultured cells were studied. Theonellamide F induced large vacuoles in 6 types of mammalian cells. The vacuoles induced by theonellamide F in 3Y1 cells accumulated acridine orange, a fluorescent probe indicating the presence of an acidic organelle. Their disappearance following treatment with bafilomycin A1 suggests that these vacuoles contain vacuolar ATPase to maintain an acidic internal milieu, and this is similar to those induced by Helicobacter pylori toxin VacA. The vacuoles induced by theonellamide F were not significantly decreased in size or number by nocodazole treatment, and the localization of a small GTPase, rab7, did not always correspond to the outline of the vacuoles. These results suggest that the molecular mode of action of vacuolar formation by theonellamides may differ from that by VacA and can be considered unique.
pubmed:language
eng
pubmed:journal
pubmed:status
PubMed-not-MEDLINE
pubmed:month
Dec
pubmed:issn
1436-2228
pubmed:author
pubmed:issnType
Print
pubmed:volume
4
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
571-82
pubmed:year
2002
pubmed:articleTitle
Accumulation of H+ in vacuoles induced by a marine peptide toxin, theonellamide F, in rat embryonic 3Y1 fibroblasts.
pubmed:affiliation
Department of Aquatic Bioscience, Graduate School of Agricultural and Life Sciences, University of Tokyo, Bunkyo, Tokyo 113-8657, Japan.
pubmed:publicationType
Journal Article