| pubmed-article:1480449 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:1480449 | lifeskim:mentions | umls-concept:C0021289 | lld:lifeskim |
| pubmed-article:1480449 | lifeskim:mentions | umls-concept:C0001675 | lld:lifeskim |
| pubmed-article:1480449 | lifeskim:mentions | umls-concept:C0007452 | lld:lifeskim |
| pubmed-article:1480449 | lifeskim:mentions | umls-concept:C0027950 | lld:lifeskim |
| pubmed-article:1480449 | lifeskim:mentions | umls-concept:C2244412 | lld:lifeskim |
| pubmed-article:1480449 | lifeskim:mentions | umls-concept:C1533691 | lld:lifeskim |
| pubmed-article:1480449 | pubmed:issue | 5 | lld:pubmed |
| pubmed-article:1480449 | pubmed:dateCreated | 1993-2-11 | lld:pubmed |
| pubmed-article:1480449 | pubmed:abstractText | Neutrophils rely on active reorganization of the cytoskeleton during movement, and functional deficiencies in the cytoskeletal elements may result in impaired neutrophil-mediated host defense. We have compared and quantitated actin polymerization in neonatal (< or = 48 h old) and adult bovine peripheral-blood polymorphonuclear leukocytes (PMN) using fluorescence flow cytometry. Baseline filamentous actin (F-actin) content of neonatal and adult PMN at time zero differed slightly but were not statistically different (p > 0.05). F-actin content of recombinant human C5a (10(-7) M)-stimulated neonatal PMN increased rapidly within 10 s of stimulation to 59.0% over baseline, then declined. F-actin in adult recombinant human C5a-stimulated PMN continued to increase for 30 s and was elevated 87.3% over baseline before subsequently declining. When stimulated with zymosan-activated bovine serum (10%), neonatal (120.7% increase) and adult PMN (115.1% increase) had similar profiles with no significant differences, and both groups reached peak F-actin levels at 30 s after stimulation. Neonatal PMN stimulated with platelet-activating factor (10(-6) M) attained peak F-actin values at 10 s (72.0% increase over baseline), but actin rapidly depolymerized by 30 s poststimulation (reduced to 29.0% increase). Adult PMN stimulated by platelet-activating factor also attained peak values by 10 s (97.6% increase over baseline), but in contrast to neonatal PMN the F-actin remained elevated at 30 s in the adult PMN (still increased 79.5%; p < 0.0.5 compared to neonatal F-actin).(ABSTRACT TRUNCATED AT 250 WORDS) | lld:pubmed |
| pubmed-article:1480449 | pubmed:language | eng | lld:pubmed |
| pubmed-article:1480449 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1480449 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:1480449 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1480449 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:1480449 | pubmed:month | Nov | lld:pubmed |
| pubmed-article:1480449 | pubmed:issn | 0031-3998 | lld:pubmed |
| pubmed-article:1480449 | pubmed:author | pubmed-author:SlausonD ODO | lld:pubmed |
| pubmed-article:1480449 | pubmed:author | pubmed-author:BochslerP NPN | lld:pubmed |
| pubmed-article:1480449 | pubmed:author | pubmed-author:NeilsenN RNR | lld:pubmed |
| pubmed-article:1480449 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:1480449 | pubmed:volume | 32 | lld:pubmed |
| pubmed-article:1480449 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:1480449 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:1480449 | pubmed:pagination | 509-13 | lld:pubmed |
| pubmed-article:1480449 | pubmed:dateRevised | 2006-11-15 | lld:pubmed |
| pubmed-article:1480449 | pubmed:meshHeading | pubmed-meshheading:1480449-... | lld:pubmed |
| pubmed-article:1480449 | pubmed:meshHeading | pubmed-meshheading:1480449-... | lld:pubmed |
| pubmed-article:1480449 | pubmed:meshHeading | pubmed-meshheading:1480449-... | lld:pubmed |
| pubmed-article:1480449 | pubmed:meshHeading | pubmed-meshheading:1480449-... | lld:pubmed |
| pubmed-article:1480449 | pubmed:meshHeading | pubmed-meshheading:1480449-... | lld:pubmed |
| pubmed-article:1480449 | pubmed:meshHeading | pubmed-meshheading:1480449-... | lld:pubmed |
| pubmed-article:1480449 | pubmed:meshHeading | pubmed-meshheading:1480449-... | lld:pubmed |
| pubmed-article:1480449 | pubmed:meshHeading | pubmed-meshheading:1480449-... | lld:pubmed |
| pubmed-article:1480449 | pubmed:year | 1992 | lld:pubmed |
| pubmed-article:1480449 | pubmed:articleTitle | Comparative actin polymerization in neonatal and adult bovine neutrophils in vitro. | lld:pubmed |
| pubmed-article:1480449 | pubmed:affiliation | Department of Pathobiology, College of Veterinary Medicine, University of Tennessee, Knoxville 37901. | lld:pubmed |
| pubmed-article:1480449 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:1480449 | pubmed:publicationType | Comparative Study | lld:pubmed |
| pubmed-article:1480449 | pubmed:publicationType | In Vitro | lld:pubmed |
| pubmed-article:1480449 | pubmed:publicationType | Research Support, U.S. Gov't, Non-P.H.S. | lld:pubmed |
| pubmed-article:1480449 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
