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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
12
|
| pubmed:dateCreated |
1993-2-11
|
| pubmed:abstractText |
The receptor for Müllerian Inhibiting Substance (MIS), a gonadal glycoprotein hormone, has not been previously identified. Plasma membranes from MIS-sensitive human tumor cell lines (HTB-111, endometrial carcinoma; and A-431, vulvar squamous carcinoma) were detergent extracted and incubated with 125I-labeled MIS anti-idiotypic antibody, or radioiodinated human recombinant MIS (125I rhMIS), with and without unlabeled competitors. 125I anti-idiotypic MIS antibody bound to HTB-111 membrane extracts was displaceable by unlabeled anti-idiotypic antibody, but not by anti-isotypic antibody prior to cross-linking. Specific binding of the anti-idiotypic MIS antibody to endometrial carcinoma cells was verified using fluorescence activated cell analysis and fluoresceinated antibody. Furthermore, unlabeled anti-idiotypic MIS antibody competed for 125I rhMIS binding to A-431 vulvar carcinoma membranes. The labeled anti-idiotypic MIS antibody binding complex could be separated from 32P labeled EGF receptor in the A-431 membranes, indicating that EGF, a natural inhibitor of MIS activity, and MIS itself bind to different receptors. These studies demonstrate a specific, displaceable binder for MIS in the plasmalemmae of two human tumor lines. Purification of this cell surface receptor protein will be greatly aided by using the MIS anti-idiotypic antibody.
|
| pubmed:grant | |
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Anti-Mullerian Hormone,
http://linkedlifedata.com/resource/pubmed/chemical/Antibodies,
http://linkedlifedata.com/resource/pubmed/chemical/Glycoproteins,
http://linkedlifedata.com/resource/pubmed/chemical/Growth Inhibitors,
http://linkedlifedata.com/resource/pubmed/chemical/Immunoglobulin Idiotypes,
http://linkedlifedata.com/resource/pubmed/chemical/Iodine Radioisotopes,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Testicular Hormones
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Dec
|
| pubmed:issn |
0018-5043
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
24
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
570-5
|
| pubmed:dateRevised |
2011-11-17
|
| pubmed:meshHeading |
pubmed-meshheading:1478615-Anti-Mullerian Hormone,
pubmed-meshheading:1478615-Antibodies,
pubmed-meshheading:1478615-Antibody Specificity,
pubmed-meshheading:1478615-Binding, Competitive,
pubmed-meshheading:1478615-Binding Sites, Antibody,
pubmed-meshheading:1478615-Carcinoma, Squamous Cell,
pubmed-meshheading:1478615-Cell Membrane,
pubmed-meshheading:1478615-Endometrial Neoplasms,
pubmed-meshheading:1478615-Female,
pubmed-meshheading:1478615-Flow Cytometry,
pubmed-meshheading:1478615-Glycoproteins,
pubmed-meshheading:1478615-Growth Inhibitors,
pubmed-meshheading:1478615-Humans,
pubmed-meshheading:1478615-Immunoglobulin Idiotypes,
pubmed-meshheading:1478615-Iodine Radioisotopes,
pubmed-meshheading:1478615-Recombinant Proteins,
pubmed-meshheading:1478615-Testicular Hormones,
pubmed-meshheading:1478615-Tumor Cells, Cultured,
pubmed-meshheading:1478615-Vulvar Neoplasms
|
| pubmed:year |
1992
|
| pubmed:articleTitle |
Identification of müllerian inhibiting substance specific binding in human cell lines.
|
| pubmed:affiliation |
Pediatric Surgical Research Laboratory, Massachusetts General Hospital, Boston.
|
| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
|