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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
6 Pt 1
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pubmed:dateCreated |
1993-2-3
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pubmed:abstractText |
We describe the development and application of a radioimmunoassay to detect circulating interleukin (IL)-1 beta concentrations in the rat. No IL-1 immunoreactivity above the detection limit of the assay (100 pg/ml) could be detected in plasma of control rats. In contrast, immunoreactive IL-1 was detected after intravenous administration of rat recombinant IL-1 beta (rrIL-1 beta) or bacterial lipopolysaccharide (LPS) to rats. The effect of LPS on plasma immunoreactive IL-1 concentrations was time and dose dependent. The immunoreactive IL-1 response to LPS was prevented by in vivo macrophage depletion induced by liposome-directed macrophage suicide technique. Gel filtration of plasma from LPS-treated rats revealed the presence of a high and a smaller molecular form of immunoreactive IL-1. The small molecular immunoreactive IL-1 peak coeluted with rrIL-1 beta and probably represents the 17-kDa form of IL-1 beta. In conclusion, our data support the hypothesis that IL-1 secreted by macrophages can act as a humoral signal molecule to induce the immunological, metabolic, and neuroendocrine changes in response to bacterial LPS.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Dec
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pubmed:issn |
0002-9513
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
263
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
E1092-8
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:1476182-Animals,
pubmed-meshheading:1476182-Antibodies,
pubmed-meshheading:1476182-Chromatography,
pubmed-meshheading:1476182-Humans,
pubmed-meshheading:1476182-Interleukin-1,
pubmed-meshheading:1476182-Lipopolysaccharides,
pubmed-meshheading:1476182-Macrophages,
pubmed-meshheading:1476182-Radioimmunoassay,
pubmed-meshheading:1476182-Rats,
pubmed-meshheading:1476182-Recombinant Proteins,
pubmed-meshheading:1476182-Titrimetry
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pubmed:year |
1992
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pubmed:articleTitle |
Development and application of a radioimmunoassay to detect interleukin-1 in rat peripheral circulation.
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pubmed:affiliation |
Department of Pharmacology, Medical Faculty, Free University, Amsterdam, The Netherlands.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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