14742670

pubmed:Citation

2

Cytosolic sulfotransferase (SULT)-mediated sulfation plays an essential role in the detoxification of bile acids and is necessary to avoid pathological conditions, such as cholestasis, liver damage, and colon cancer. In this study, using transgenic mice bearing conditional expression of the activated constitutive androstane receptor (CAR), we demonstrate that activation of CAR is both necessary and sufficient to confer resistance to the hepatotoxicity of lithocholic acid (LCA). Surprisingly, the CAR-mediated protection is not attributable to the expected and previously characterized CYP3A pathway; rather, it is associated with a robust induction of SULT gene expression and increased LCA sulfation. We have also provided direct evidence that CAR regulates SULT expression by binding to the CAR response elements found within the SULT gene promoters. Interestingly, activation of CAR was also associated with an increased expression of the 3'-phosphoadenosine 5'-phosphosulfate synthetase 2 (PAPSS2), an enzyme responsible for generating the sulfate donor 3'-phosphoadenosine-5'-phosphosulfate. Analysis of gene knockout mice revealed that CAR is also indispensable for ligand-dependent activation of SULT and PAPSS2 in vivo. Therefore, we establish an essential and unique role of CAR in controlling the mammalian sulfation system and its implication in the detoxification of bile acids.

eng

IM

MEDLINE

0026-895X

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NLM

292-300

pubmed-meshheading:14742670-Animals, pubmed-meshheading:14742670-Aryl Hydrocarbon Hydroxylases, pubmed-meshheading:14742670-Arylsulfotransferase, pubmed-meshheading:14742670-Bile Acids and Salts, pubmed-meshheading:14742670-Cytochrome P-450 CYP3A, pubmed-meshheading:14742670-Female, pubmed-meshheading:14742670-Lithocholic Acid, pubmed-meshheading:14742670-Liver, pubmed-meshheading:14742670-Male, pubmed-meshheading:14742670-Metabolic Detoxication, Drug, pubmed-meshheading:14742670-Mice, pubmed-meshheading:14742670-Mice, Transgenic, pubmed-meshheading:14742670-Multienzyme Complexes, pubmed-meshheading:14742670-Oxidoreductases, N-Demethylating, pubmed-meshheading:14742670-Receptors, Cytoplasmic and Nuclear, pubmed-meshheading:14742670-Signal Transduction, pubmed-meshheading:14742670-Sulfate Adenylyltransferase, pubmed-meshheading:14742670-Sulfotransferases, pubmed-meshheading:14742670-Transcription Factors

A novel constitutive androstane receptor-mediated and CYP3A-independent pathway of bile acid detoxification.

Journal Article, Comparative Study, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't