Source:http://linkedlifedata.com/resource/pubmed/id/14735144
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
1
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| pubmed:dateCreated |
2004-1-21
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| pubmed:abstractText |
Association studies have identified the interleukin-1 receptor antagonist gene allele 2(IL-1RN*2) as a marker of susceptibility in ulcerative colitis (UC). This study investigated the significance of the IL-1RN genotype with respect to protein and mRNA expression in the colonic mucosa. Homogenates of rectal biopsies from 99 UC and 54 controls were assayed for cytokines IL-1ra, IL-1a and IL-1b using ELISA. IL1RN, IL1A and IL1B genotypes were determined using restriction-enzyme analysis. The ability of the two IL1RN alleles to generate steady-state mRNA accumulation was assessed in the colonic mucosa of seven heterozygous patients. Stepwise linear regression demonstrated that IL-1RN genotype (P=0.001), diagnosis (P<0.0001) and treatment (P<0.03) were independent factors associated with the IL-1ra protein level whilst IL1RN genotype (P=0.005) and macroscopic inflammatory grade (P<0.0001) were associated with the IL-1ra/ total IL-1 ratio. The IL1RN*2 correlated with reduced IL-1ra and IL-1ra/IL-1 ratio with a gene dosage effect. In heterozygous UC patients the ratio of allele 1 mRNA / allele 2 steady state mRNA was always greater than 1 (range: 1.2-3.1) (P=0.018). The IL-1RN*2 is associated with reduced levels of IL-1ra protein and IL-1RN mRNA in the colonic mucosa, providing a biologically plausible explanation for the observed association of the allele with the disease.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/3' Untranslated Regions,
http://linkedlifedata.com/resource/pubmed/chemical/DNA, Complementary,
http://linkedlifedata.com/resource/pubmed/chemical/IL1RN protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin 1 Receptor Antagonist...,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-1,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Sialoglycoproteins
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| pubmed:status |
MEDLINE
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| pubmed:month |
Jan
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| pubmed:issn |
1466-4879
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| pubmed:author | |
| pubmed:issnType |
Print
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| pubmed:volume |
5
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
8-15
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| pubmed:dateRevised |
2006-11-15
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| pubmed:meshHeading |
pubmed-meshheading:14735144-3' Untranslated Regions,
pubmed-meshheading:14735144-Adolescent,
pubmed-meshheading:14735144-Adult,
pubmed-meshheading:14735144-Aged,
pubmed-meshheading:14735144-Aged, 80 and over,
pubmed-meshheading:14735144-Alleles,
pubmed-meshheading:14735144-Child,
pubmed-meshheading:14735144-Colitis, Ulcerative,
pubmed-meshheading:14735144-DNA, Complementary,
pubmed-meshheading:14735144-Female,
pubmed-meshheading:14735144-Genotype,
pubmed-meshheading:14735144-Humans,
pubmed-meshheading:14735144-Interleukin 1 Receptor Antagonist Protein,
pubmed-meshheading:14735144-Interleukin-1,
pubmed-meshheading:14735144-Intestinal Mucosa,
pubmed-meshheading:14735144-Male,
pubmed-meshheading:14735144-Middle Aged,
pubmed-meshheading:14735144-Polymorphism, Genetic,
pubmed-meshheading:14735144-RNA, Messenger,
pubmed-meshheading:14735144-Sialoglycoproteins
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| pubmed:year |
2004
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| pubmed:articleTitle |
Functional correlates of the interleukin-1 receptor antagonist gene polymorphism in the colonic mucosa in ulcerative colitis.
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| pubmed:affiliation |
The Gastroenterology and Liver Unit, University of Sheffield, Royal Hallamshire Hospital, Sheffield, UK. Martyn.J.Carter@btinternet.com
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| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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