Source:http://linkedlifedata.com/resource/pubmed/id/14733494
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
2
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pubmed:dateCreated |
2004-1-21
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pubmed:abstractText |
Corn silage was dried, ground, and then extracted with 0.1 M ethylenediaminetetraacetic acid. The filtrate was applied to a FumoniTest immunoaffinity column. Fumonisins were derivatized with naphthalene-2,3-dicarboxaldehyde, separated on a C(18) liquid chromatographic column, and detected by fluorescence. The detection limits for fumonisin B(1), fumonisin B(2), and fumonisin B(3) were 50, 25, and 25 ng/g of dried silage, respectively. Recoveries of fumonisin B(1), fumonisin B(2), and fumonisin B(3) from wet and dried corn silage spiked over the range of 100-5000 ng/g averaged 91-106%. The method was applied to corn silage samples collected from the midwestern area of the United States during 2001-2002. Of 89 corn silage samples, fumonisin B(1), fumonisin B(2), and fumonisin B(3) were found in 86 (97%), 64 (72%), and 51 (57%) of the samples. The mean positive levels of fumonisin B(1), fumonisin B(2), and fumonisin B(3) were 615, 93, and 51 ng/g, respectively, in dried silage. This suggests that fumonisins may be frequent low level contaminants in corn silage.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/2,3-naphthalenedicarboxaldehyde,
http://linkedlifedata.com/resource/pubmed/chemical/Fumonisins,
http://linkedlifedata.com/resource/pubmed/chemical/Naphthalenes,
http://linkedlifedata.com/resource/pubmed/chemical/Solvents,
http://linkedlifedata.com/resource/pubmed/chemical/fumonisin B1,
http://linkedlifedata.com/resource/pubmed/chemical/fumonisin B2,
http://linkedlifedata.com/resource/pubmed/chemical/fumonisin B3
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pubmed:status |
MEDLINE
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pubmed:month |
Jan
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pubmed:issn |
0021-8561
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:day |
28
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pubmed:volume |
52
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
196-200
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pubmed:meshHeading |
pubmed-meshheading:14733494-Chromatography, High Pressure Liquid,
pubmed-meshheading:14733494-Food Contamination,
pubmed-meshheading:14733494-Fumonisins,
pubmed-meshheading:14733494-Naphthalenes,
pubmed-meshheading:14733494-Silage,
pubmed-meshheading:14733494-Solvents,
pubmed-meshheading:14733494-Zea mays
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pubmed:year |
2004
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pubmed:articleTitle |
Liquid chromatographic determination of fumonisins B1, B2, and B3 in corn silage.
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pubmed:affiliation |
Mycotoxin Research Unit, National Center for Agricultural Utilization Research, USDA/ARS, 1815 North University Street, Peoria, Illinois 61604, USA.
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pubmed:publicationType |
Journal Article
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