Source:http://linkedlifedata.com/resource/pubmed/id/14733135
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
5
|
| pubmed:dateCreated |
2004-1-21
|
| pubmed:abstractText |
Inter-laboratory evaluation studies were conducted for the notified ELISA methods for allergic substances (Egg). Standard extracts of egg spiked in extracts of sausage, sauce, cookie, bread and cereal at a level of 5-20 ng/mL as the sample solution were analyzed in replicate in 10 laboratories. Coefficients of variation (CVs) of all three ELISA methods using an Egg Protein ovalbumin ELISA Kit (ovalbumin kit), an Egg Protein ovomucoid ELISA Kit (ovomucoid kit) and a FASTKIT Egg ELISA kit (Egg ELISA kit) were mostly less than 10%. Mean recoveries of the standard extract of egg were over 40% in the three ELISA methods. Repeatability relative standard deviations of egg standard solution in five food extracts were in the ranges of 18.7-25.5%, 18.6-41.8%, 21.3-43.3% for the ovalbumin kit, the ovomucoid kit and the Egg ELISA kit, respectively. Reproducibility relative standard deviations of egg standard solution in five food extracts were 16.8-35.1%, 19.6-35.8%, 24.7-51.1% for the ovalbumin kit, the ovomucoid kit and the Egg ELISA kit, respectively. The detection limits of all the ELISA methods were 4-5 ng/mL in sample solutions. These results suggested that the notified ELISA methods are reliable and reproducible for the inspection of egg protein levels in extracts of sausage, sauce, cookie, bread and cereal.
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| pubmed:language |
jpn
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Oct
|
| pubmed:issn |
0015-6426
|
| pubmed:author |
pubmed-author:AkiyamaHiroshiH,
pubmed-author:ArakawaFumihiroF,
pubmed-author:HarikaiNaokiN,
pubmed-author:HonjohTsutomuT,
pubmed-author:IijimaKenK,
pubmed-author:ImamuraTomoakiT,
pubmed-author:IsuzugawaKazutoK,
pubmed-author:KatoHisashiH,
pubmed-author:MaitaniTamioT,
pubmed-author:MamegoshiShinichiS,
pubmed-author:MizuguchiYamatoY,
pubmed-author:MorimatsuFumikiF,
pubmed-author:MuraokaShirooS,
pubmed-author:NishiharaRikukaR,
pubmed-author:OgasawaraTakeshiT,
pubmed-author:SatoHidetakaH,
pubmed-author:TakahataYoshihisaY,
pubmed-author:ToyodaMasatakeM,
pubmed-author:WakuiChisekoC,
pubmed-author:WataiMasatoshiM,
pubmed-author:WatanabeHirokoH,
pubmed-author:WatanabeTakahiroT,
pubmed-author:YamakawaHirohitoH,
pubmed-author:YamamotoMihoM,
pubmed-author:YamauchiAtsushiA,
pubmed-author:YoshikawaReijiR
|
| pubmed:issnType |
Print
|
| pubmed:volume |
44
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
213-9
|
| pubmed:dateRevised |
2006-11-15
|
| pubmed:meshHeading | |
| pubmed:year |
2003
|
| pubmed:articleTitle |
[Inter-laboratory evaluation studies of notified ELISA methods for allergic substances (egg)].
|
| pubmed:affiliation |
National Institute of Health Sciences: 1-18-1, Kamiyoga, Setagaya-ku, Tokyo 158-8501, Japan.
|
| pubmed:publicationType |
Journal Article,
English Abstract,
Research Support, Non-U.S. Gov't,
Multicenter Study
|