Source:http://linkedlifedata.com/resource/pubmed/id/14724072
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
1
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| pubmed:dateCreated |
2004-1-15
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| pubmed:abstractText |
We previously reported that aortic smooth muscle cells (SMC) from aged mice have an age-related decline in proliferative capacity compared with those derived from young mice. Here we investigated matrix metalloproteinase-9 (MMP-9) regulation in both young and aged SMC. Zymography, immunoblot, and northern blot analysis showed that MMP-9 expression is significantly reduced in response to tumor necrosis factor-alpha stimulation with increasing in vitro age. Mutational analysis, gel shift assays and supershift assays demonstrated that the lower MMP-9 expression in aged SMC is associated with lower activities of NF-kappaB and AP-1. Since mitogen-activated protein kinase ERK1/2 induce MMP-9 expression, we examined whether U0126, an ERK1/2 inhibitor, influenced MMP-9 expression in aged SMC. Treatment with U0126 successfully inhibited MMP-9 expression in both young and aged SMC. Finally, to analyze the causal relationship between replicative senescence and MMP-9 expression, we stably overexpressed the MMP-9 gene in aged SMC and we showed no alteration of the proliferative capacity of the transduced cells. Taken together, these results suggest that down-regulation of MMP-9 expression in SMC may play a role in vascular remodeling during in vitro aging.
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| pubmed:grant | |
| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical | |
| pubmed:status |
MEDLINE
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| pubmed:month |
Jan
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| pubmed:issn |
0531-5565
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| pubmed:author | |
| pubmed:issnType |
Print
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| pubmed:volume |
39
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
123-31
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| pubmed:dateRevised |
2008-11-21
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| pubmed:meshHeading |
pubmed-meshheading:14724072-Aging,
pubmed-meshheading:14724072-Animals,
pubmed-meshheading:14724072-Aorta,
pubmed-meshheading:14724072-Blotting, Northern,
pubmed-meshheading:14724072-Cell Division,
pubmed-meshheading:14724072-Cells, Cultured,
pubmed-meshheading:14724072-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:14724072-Gene Expression,
pubmed-meshheading:14724072-Immunoblotting,
pubmed-meshheading:14724072-Male,
pubmed-meshheading:14724072-Matrix Metalloproteinase 9,
pubmed-meshheading:14724072-Mice,
pubmed-meshheading:14724072-Muscle, Smooth,
pubmed-meshheading:14724072-NF-kappa B,
pubmed-meshheading:14724072-Promoter Regions, Genetic,
pubmed-meshheading:14724072-Transcription Factor AP-1,
pubmed-meshheading:14724072-Transduction, Genetic,
pubmed-meshheading:14724072-Tumor Necrosis Factor-alpha
|
| pubmed:year |
2004
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| pubmed:articleTitle |
Age-related changes in matrix metalloproteinase-9 regulation in cultured mouse aortic smooth muscle cells.
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| pubmed:affiliation |
National Research Laboratory for Glycobiology, Korean Ministry of Science and Technology, Kyungju, Kyungbuk 780-714, South Korea.
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| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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