Linked Life Data

14719166

Source:http://linkedlifedata.com/resource/pubmed/id/14719166

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
2004-1-13
pubmed:abstractText
A marine bacterial strain, Fucobacter marina, produced extracellular sulfated fucoglucuronomannan (SFGM) lyase when cultivated in the presence of crude SFGM obtained from fucoidan of Kjellmaniella crassifolia (brown algae) by cetyl pyridinium chloride fractionation. For the SFGM lyase assay, SFGM fraction separated from K. crassifolia fucoidan by anion exchange column chromatography was used as the substrate. The extracellular SFGM lyase was purified to homogeneity on an electrophoresis gel with 4240-fold purity at 13.8% yield. The enzyme proved to be a monomer, since gel filtration and sodium dodecyl sulfate polyacrylamide gel electrophoresis gave the same relative molecular mass of 67,000. The enzyme specifically digested SFGM but did not digest any other uronic-acid-containing polysaccharides tested. The optimum conditions for the enzyme reaction were around pH 7.5, 43 degrees C, and 0.4 M NaCl concentration. The enzyme was strongly inhibited by CuCl(2) and ZnCl(2), and also by some sulfhydryl reagents.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
1436-2228
pubmed:author
pubmed:issnType
Print
pubmed:volume
5
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
380-7
pubmed:dateRevised
2010-11-18
pubmed:meshHeading
pubmed:articleTitle
Purification of sulfated fucoglucuronomannan lyase from bacterial strain of Fucobacter marina and study of appropriate conditions for its enzyme digestion.
pubmed:affiliation
Takara Bio Inc., Biotechnology Research Laboratories, Zaifu-cho 82-4, Hirosaki-shi, Aomori-ken, 036-8216, Japan. sakait@takara-bio.co.jp
pubmed:publicationType
Journal Article