| pubmed-article:14718262 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:14718262 | lifeskim:mentions | umls-concept:C0040300 | lld:lifeskim |
| pubmed-article:14718262 | lifeskim:mentions | umls-concept:C0596901 | lld:lifeskim |
| pubmed-article:14718262 | lifeskim:mentions | umls-concept:C0221464 | lld:lifeskim |
| pubmed-article:14718262 | lifeskim:mentions | umls-concept:C0034783 | lld:lifeskim |
| pubmed-article:14718262 | lifeskim:mentions | umls-concept:C0597357 | lld:lifeskim |
| pubmed-article:14718262 | lifeskim:mentions | umls-concept:C1979844 | lld:lifeskim |
| pubmed-article:14718262 | lifeskim:mentions | umls-concept:C0022702 | lld:lifeskim |
| pubmed-article:14718262 | lifeskim:mentions | umls-concept:C1167622 | lld:lifeskim |
| pubmed-article:14718262 | lifeskim:mentions | umls-concept:C0220825 | lld:lifeskim |
| pubmed-article:14718262 | lifeskim:mentions | umls-concept:C1707455 | lld:lifeskim |
| pubmed-article:14718262 | lifeskim:mentions | umls-concept:C0205266 | lld:lifeskim |
| pubmed-article:14718262 | pubmed:issue | 3 | lld:pubmed |
| pubmed-article:14718262 | pubmed:dateCreated | 2004-2-6 | lld:pubmed |
| pubmed-article:14718262 | pubmed:abstractText | The binding kinetics of [3H]-prazosin were measured using intact segments of rat tail artery (RTA) and thoracic aorta (RAO), and the data were compared with those obtained using a conventional membrane ligand-binding method. In intact RTA and RAO segments, [3H]-prazosin bound reversibly in a time-dependent and receptor-specific manner at 4 degrees C to alpha-1 adrenoceptors (ARs) of the plasma membrane, with affinities (pKD): 9.5 in RTA; 9.9 in RAO) that were in agreement with values estimated by a conventional membrane ligand-binding method. However, nonspecific binding was considerably higher in RAO than RTA, failing to detect clearly the specific binding at high concentrations (>300 pm) of [3H]-prazosin in binding experiments with RAO segments and membranes. The abundance of receptor in the RTA and RAO (Bmax mg-1) of total tissue protein), estimated using the tissue segment-binding approach (527+/-14 fmol mg-1 for RTA; 138+/-4 fmol mg-1 for RAO), was about 25-fold higher than values estimated using a conventional membrane-binding method (22+/-5 fmol mg-1) for RTA; 5+/-1 fmol mg-1 for RAO). Binding competition experiments using intact tissue segments or membranes derived from RTA tissue yielded comparable data, indicating a coexistence of alpha-1A AR (high affinity for prazosin, KMD-3213 and WB4101 and low affinity for BMY 7378) and alpha-1B AR (high affinity for prazosin but low affinity for KMD-3213, WB4101 and BMY 7378). In RAO tissue, careful evaluation of the tissue segment-binding assay revealed the coexpression of alpha-1B AR (high affinity for prazosin, but low affinity for KMD-3213 and BMY 7378) and alpha-1D AR (high affinity for prazosin and BMY 7378, but low affinity for KMD-3213), whereas the membrane-binding approach failed to detect these receptor subtypes with certainty. The present study indicates that previous estimates of alpha-1 AR density and alpha-1 AR subtypes obtained by a conventional membrane-binding approach, as opposed to our improved tissue segment-binding assay, may have substantially underestimated the abundance of receptors present in arterial tissues, and may have failed to identify accurately the presence of receptor subtypes. Advantages and disadvantages of the tissue segment-binding approach are discussed.British Journal of Pharmacology (2004) 141, 468-476. doi:10.1038/sj.bjp.0705627 | lld:pubmed |
| pubmed-article:14718262 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:14718262 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:14718262 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:14718262 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:14718262 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:14718262 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:14718262 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:14718262 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:14718262 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:14718262 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:14718262 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:14718262 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:14718262 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:14718262 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:14718262 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:14718262 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:14718262 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:14718262 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:14718262 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:14718262 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:14718262 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:14718262 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:14718262 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:14718262 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:14718262 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:14718262 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:14718262 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:14718262 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:14718262 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:14718262 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:14718262 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:14718262 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:14718262 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:14718262 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:14718262 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:14718262 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:14718262 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:14718262 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:14718262 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:14718262 | pubmed:language | eng | lld:pubmed |
| pubmed-article:14718262 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:14718262 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:14718262 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:14718262 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:14718262 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:14718262 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:14718262 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:14718262 | pubmed:month | Feb | lld:pubmed |
| pubmed-article:14718262 | pubmed:issn | 0007-1188 | lld:pubmed |
| pubmed-article:14718262 | pubmed:author | pubmed-author:SuzukiFumikoF | lld:pubmed |
| pubmed-article:14718262 | pubmed:author | pubmed-author:ZhangLiL | lld:pubmed |
| pubmed-article:14718262 | pubmed:author | pubmed-author:TanakaTakashi... | lld:pubmed |
| pubmed-article:14718262 | pubmed:author | pubmed-author:MuramatsuIkun... | lld:pubmed |
| pubmed-article:14718262 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:14718262 | pubmed:volume | 141 | lld:pubmed |
| pubmed-article:14718262 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:14718262 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:14718262 | pubmed:pagination | 468-76 | lld:pubmed |
| pubmed-article:14718262 | pubmed:dateRevised | 2010-11-18 | lld:pubmed |
| pubmed-article:14718262 | pubmed:meshHeading | pubmed-meshheading:14718262... | lld:pubmed |
| pubmed-article:14718262 | pubmed:meshHeading | pubmed-meshheading:14718262... | lld:pubmed |
| pubmed-article:14718262 | pubmed:meshHeading | pubmed-meshheading:14718262... | lld:pubmed |
| pubmed-article:14718262 | pubmed:meshHeading | pubmed-meshheading:14718262... | lld:pubmed |
| pubmed-article:14718262 | pubmed:meshHeading | pubmed-meshheading:14718262... | lld:pubmed |
| pubmed-article:14718262 | pubmed:meshHeading | pubmed-meshheading:14718262... | lld:pubmed |
| pubmed-article:14718262 | pubmed:meshHeading | pubmed-meshheading:14718262... | lld:pubmed |
| pubmed-article:14718262 | pubmed:meshHeading | pubmed-meshheading:14718262... | lld:pubmed |
| pubmed-article:14718262 | pubmed:meshHeading | pubmed-meshheading:14718262... | lld:pubmed |
| pubmed-article:14718262 | pubmed:meshHeading | pubmed-meshheading:14718262... | lld:pubmed |
| pubmed-article:14718262 | pubmed:meshHeading | pubmed-meshheading:14718262... | lld:pubmed |
| pubmed-article:14718262 | pubmed:meshHeading | pubmed-meshheading:14718262... | lld:pubmed |
| pubmed-article:14718262 | pubmed:meshHeading | pubmed-meshheading:14718262... | lld:pubmed |
| pubmed-article:14718262 | pubmed:meshHeading | pubmed-meshheading:14718262... | lld:pubmed |
| pubmed-article:14718262 | pubmed:meshHeading | pubmed-meshheading:14718262... | lld:pubmed |
| pubmed-article:14718262 | pubmed:year | 2004 | lld:pubmed |
| pubmed-article:14718262 | pubmed:articleTitle | Alpha-1 adrenoceptors: evaluation of receptor subtype-binding kinetics in intact arterial tissues and comparison with membrane binding. | lld:pubmed |
| pubmed-article:14718262 | pubmed:affiliation | Department of Pharmacology, School of Medicine, Fukui Medical University, Matsuoka, Fukui 910-1193, Japan. | lld:pubmed |
| pubmed-article:14718262 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:14718262 | pubmed:publicationType | Comparative Study | lld:pubmed |
| pubmed-article:14718262 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:14718262 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:14718262 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:14718262 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:14718262 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:14718262 | lld:pubmed |
