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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
2004-1-12
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pubmed:abstractText |
In this report, we studied the functional characteristics of a brain peptide transporter using synaptosomes prepared from rat cerebral cortex. Crude synaptosomes (P(2) fraction) were prepared from cerebral cortices in male Wistar rats. Uptake of [14C]glycylsarcosine (Gly-Sar), a substrate for H(+)/oligopeptide transporters PEPT1 and PEPT2, and [3H]histidine, a substrate for peptide/histidine transporters PHT1 and PHT2, was measured at 37 degrees C by a rapid filtration technique. The uptake of [14C]Gly-Sar into synaptosomes was stimulated by an inwardly directed H(+)-gradient. The uptake system exhibited a Michaelis-Menten constant (K(t)) of 110+/-20 microM for Gly-Sar. This value is comparable to the K(t) value for Gly-Sar uptake via the high-affinity H(+)/peptide transporter PEPT2. The H(+)-dependent uptake of [14C]Gly-Sar into synaptosomes was inhibited by di- and tripeptides and beta-lactam antibiotics, but was unaffected by amino acids glycine and histidine. In particular, kyotorphin (Tyr-Arg) completely inhibited Gly-Sar uptake with the K(i) value of 29+/-14 microM. These uptake properties of the brain peptide transporter (i.e., the K(t) value for Gly-Sar uptake and the K(i) value of kyotorphin for Gly-Sar uptake) are very similar to those of PEPT2. RT-PCR and Western blotting analyses revealed that PEPT2 is actually expressed in the cerebral cortex in rat. These results indicate that a H(+)-coupled high affinity peptide transport system is functionally expressed in the cerebral cortex and that this transport system is identical to PEPT2.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Anti-Bacterial Agents,
http://linkedlifedata.com/resource/pubmed/chemical/Carbon Isotopes,
http://linkedlifedata.com/resource/pubmed/chemical/Carrier Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Dipeptides,
http://linkedlifedata.com/resource/pubmed/chemical/Histidine,
http://linkedlifedata.com/resource/pubmed/chemical/Membrane Transport Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Nerve Tissue Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/PepT1 protein,
http://linkedlifedata.com/resource/pubmed/chemical/Pht2 protein, rat,
http://linkedlifedata.com/resource/pubmed/chemical/Protons,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Slc15a1 protein, rat,
http://linkedlifedata.com/resource/pubmed/chemical/Slc15a4 protein, rat,
http://linkedlifedata.com/resource/pubmed/chemical/Symporters,
http://linkedlifedata.com/resource/pubmed/chemical/Tritium,
http://linkedlifedata.com/resource/pubmed/chemical/glycylsarcosine,
http://linkedlifedata.com/resource/pubmed/chemical/hydrogen-coupled oligopeptide...
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pubmed:status |
MEDLINE
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pubmed:month |
Jan
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pubmed:issn |
0006-8993
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
30
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pubmed:volume |
997
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
52-61
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pubmed:dateRevised |
2007-11-14
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pubmed:meshHeading |
pubmed-meshheading:14715149-Animals,
pubmed-meshheading:14715149-Anti-Bacterial Agents,
pubmed-meshheading:14715149-Biological Transport,
pubmed-meshheading:14715149-Blotting, Northern,
pubmed-meshheading:14715149-Blotting, Western,
pubmed-meshheading:14715149-Carbon Isotopes,
pubmed-meshheading:14715149-Carrier Proteins,
pubmed-meshheading:14715149-Cerebral Cortex,
pubmed-meshheading:14715149-Dipeptides,
pubmed-meshheading:14715149-Dose-Response Relationship, Drug,
pubmed-meshheading:14715149-Histidine,
pubmed-meshheading:14715149-Kinetics,
pubmed-meshheading:14715149-Male,
pubmed-meshheading:14715149-Membrane Transport Proteins,
pubmed-meshheading:14715149-Nerve Tissue Proteins,
pubmed-meshheading:14715149-Protons,
pubmed-meshheading:14715149-RNA, Messenger,
pubmed-meshheading:14715149-Rats,
pubmed-meshheading:14715149-Rats, Wistar,
pubmed-meshheading:14715149-Reverse Transcriptase Polymerase Chain Reaction,
pubmed-meshheading:14715149-Substrate Specificity,
pubmed-meshheading:14715149-Symporters,
pubmed-meshheading:14715149-Synaptosomes,
pubmed-meshheading:14715149-Time Factors,
pubmed-meshheading:14715149-Tritium
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pubmed:year |
2004
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pubmed:articleTitle |
Functional characterization of brain peptide transporter in rat cerebral cortex: identification of the high-affinity type H+/peptide transporter PEPT2.
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pubmed:affiliation |
Department of Biochemical Pharmacology, Kyoto Pharmaceutical University, Yamashina, Kyoto 607-8414, Japan. fujita@mb.kyoto-phu.ac.jp
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pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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