Source:http://linkedlifedata.com/resource/pubmed/id/14712275
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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
6970
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pubmed:dateCreated |
2004-1-8
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pubmed:abstractText |
Primary T-cell responses in lymph nodes (LNs) require contact-dependent information exchange between T cells and dendritic cells (DCs). Because lymphocytes continually enter and leave normal LNs, the resident lymphocyte pool is composed of non-synchronized cells with different dwell times that display heterogeneous behaviour in mouse LNs in vitro. Here we employ two-photon microscopy in vivo to study antigen-presenting DCs and naive T cells whose dwell time in LNs was synchronized. During the first 8 h after entering from the blood, T cells underwent multiple short encounters with DCs, progressively decreased their motility, and upregulated activation markers. During the subsequent 12 h T cells formed long-lasting stable conjugates with DCs and began to secrete interleukin-2 and interferon-gamma. On the second day, coinciding with the onset of proliferation, T cells resumed their rapid migration and short DC contacts. Thus, T-cell priming by DCs occurs in three successive stages: transient serial encounters during the first activation phase are followed by a second phase of stable contacts culminating in cytokine production, which makes a transition into a third phase of high motility and rapid proliferation.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Jan
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pubmed:issn |
1476-4687
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pubmed:author | |
pubmed:issnType |
Electronic
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pubmed:day |
8
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pubmed:volume |
427
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
154-9
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:14712275-Amino Acid Sequence,
pubmed-meshheading:14712275-Animals,
pubmed-meshheading:14712275-Cell Division,
pubmed-meshheading:14712275-Cell Movement,
pubmed-meshheading:14712275-Cells, Cultured,
pubmed-meshheading:14712275-Dendritic Cells,
pubmed-meshheading:14712275-Interferon-gamma,
pubmed-meshheading:14712275-Interleukin-2,
pubmed-meshheading:14712275-Lymph Nodes,
pubmed-meshheading:14712275-Lymphocyte Activation,
pubmed-meshheading:14712275-Mice,
pubmed-meshheading:14712275-Mice, Inbred BALB C,
pubmed-meshheading:14712275-Mice, Inbred C57BL,
pubmed-meshheading:14712275-Molecular Sequence Data,
pubmed-meshheading:14712275-T-Lymphocytes,
pubmed-meshheading:14712275-Time Factors
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pubmed:year |
2004
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pubmed:articleTitle |
T-cell priming by dendritic cells in lymph nodes occurs in three distinct phases.
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pubmed:affiliation |
The CBR Institute for Biomedical Research, Department of Pathology, Harvard Medical School, 200 Longwood Avenue, Boston, Massachusetts 02115, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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