Linked Life Data

14711691

Source:http://linkedlifedata.com/resource/pubmed/id/14711691

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2004-1-8
pubmed:databankReference
pubmed:abstractText
A denitrifying microbial consortium was enriched in an anoxically operated, methanol-fed sequencing batch reactor (SBR) fed with a mineral salts medium containing methanol as the sole carbon source and nitrate as the electron acceptor. The SBR was inoculated with sludge from a biological nutrient removal activated sludge plant exhibiting good denitrification. The SBR denitrification rate improved from less than 0.02 mg of NO(3)(-)-N mg of mixed-liquor volatile suspended solids (MLVSS)(-1) h(-1) to a steady-state value of 0.06 mg of NO(3)(-)-N mg of MLVSS(-1) h(-1) over a 7-month operational period. At this time, the enriched microbial community was subjected to stable-isotope probing (SIP) with [(13)C]methanol to biomark the DNA of the denitrifiers. The extracted [(13)C]DNA and [(12)C]DNA from the SIP experiment were separately subjected to full-cycle rRNA analysis. The dominant 16S rRNA gene phylotype (group A clones) in the [(13)C]DNA clone library was closely related to those of the obligate methylotrophs Methylobacillus and Methylophilus in the order Methylophilales of the Betaproteobacteria (96 to 97% sequence identities), while the most abundant clone groups in the [(12)C]DNA clone library mostly belonged to the family Saprospiraceae in the Bacteroidetes phylum. Oligonucleotide probes for use in fluorescence in situ hybridization (FISH) were designed to specifically target the group A clones and Methylophilales (probes DEN67 and MET1216, respectively) and the Saprospiraceae clones (probe SAP553). Application of these probes to the SBR biomass over the enrichment period demonstrated a strong correlation between the level of SBR denitrification and relative abundance of DEN67-targeted bacteria in the SBR community. By contrast, there was no correlation between the denitrification rate and the relative abundances of the well-known denitrifying genera Hyphomicrobium and Paracoccus or the Saprospiraceae clones visualized by FISH in the SBR biomass. FISH combined with microautoradiography independently confirmed that the DEN67-targeted cells were the dominant bacterial group capable of anoxic [(14)C]methanol uptake in the enriched biomass. The well-known denitrification lag period in the methanol-fed SBR was shown to coincide with a lag phase in growth of the DEN67-targeted denitrifying population. We conclude that Methylophilales bacteria are the dominant denitrifiers in our SBR system and likely are important denitrifiers in full-scale methanol-fed denitrifying sludges.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/14711691-10049895, http://linkedlifedata.com/resource/pubmed/commentcorrection/14711691-10473419, http://linkedlifedata.com/resource/pubmed/commentcorrection/14711691-10553296, http://linkedlifedata.com/resource/pubmed/commentcorrection/14711691-10688198, http://linkedlifedata.com/resource/pubmed/commentcorrection/14711691-10698787, http://linkedlifedata.com/resource/pubmed/commentcorrection/14711691-10698788, http://linkedlifedata.com/resource/pubmed/commentcorrection/14711691-10879983, http://linkedlifedata.com/resource/pubmed/commentcorrection/14711691-12520066, http://linkedlifedata.com/resource/pubmed/commentcorrection/14711691-12768619, http://linkedlifedata.com/resource/pubmed/commentcorrection/14711691-2200342, http://linkedlifedata.com/resource/pubmed/commentcorrection/14711691-2231712, http://linkedlifedata.com/resource/pubmed/commentcorrection/14711691-2430518, http://linkedlifedata.com/resource/pubmed/commentcorrection/14711691-7028991, http://linkedlifedata.com/resource/pubmed/commentcorrection/14711691-7535888, http://linkedlifedata.com/resource/pubmed/commentcorrection/14711691-8953706, http://linkedlifedata.com/resource/pubmed/commentcorrection/14711691-9572966, http://linkedlifedata.com/resource/pubmed/commentcorrection/14711691-9758812
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
0099-2240
pubmed:author
pubmed:issnType
Print
pubmed:volume
70
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
588-96
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
2004
pubmed:articleTitle
Use of stable-isotope probing, full-cycle rRNA analysis, and fluorescence in situ hybridization-microautoradiography to study a methanol-fed denitrifying microbial community.
pubmed:affiliation
Advanced Wastewater Management Centre, The University of Queensland, St. Lucia 4072, Queensland, Australia.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't