| pubmed-article:14688405 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:14688405 | lifeskim:mentions | umls-concept:C0003250 | lld:lifeskim |
| pubmed-article:14688405 | lifeskim:mentions | umls-concept:C0079411 | lld:lifeskim |
| pubmed-article:14688405 | pubmed:issue | 1 | lld:pubmed |
| pubmed-article:14688405 | pubmed:dateCreated | 2004-1-7 | lld:pubmed |
| pubmed-article:14688405 | pubmed:abstractText | Production of monoclonal antibodies requires immortalization of splenocytes by somatic fusion to a myeloma cell line partner (hybridomas). Although hybridomas can be immortal, they may depend on a feeder cell layer and may be genetically unstable. Since the inception of hybridoma technology, efforts to improve efficiency and stability of monoclonal antibody-producing cell lines have not brought about substantial progress. Moreover, suitable human multiple myeloma-derived cell lines for the production of human antibodies have been very difficult to develop. Here we report a strategy that greatly simplifies the generation of antibodies and eliminates the need for hybridomas. We show that splenocytes derived from transgenic mice harboring a mutant temperature-sensitive simian virus 40 large tumor antigen under the control of a mouse major histocompatibility promoter are conditionally immortal at permissive temperatures and produce monoclonal antibodies. This simple approach may become a method of choice for generation and production of both polyclonal and monoclonal antibodies with advantages in high-throughput discovery and antibody-based immunotherapy. | lld:pubmed |
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| pubmed-article:14688405 | pubmed:language | eng | lld:pubmed |
| pubmed-article:14688405 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:14688405 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:14688405 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:14688405 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
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| pubmed-article:14688405 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:14688405 | pubmed:month | Jan | lld:pubmed |
| pubmed-article:14688405 | pubmed:issn | 0027-8424 | lld:pubmed |
| pubmed-article:14688405 | pubmed:author | pubmed-author:ArapWadihW | lld:pubmed |
| pubmed-article:14688405 | pubmed:author | pubmed-author:PasqualiniRen... | lld:pubmed |
| pubmed-article:14688405 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:14688405 | pubmed:day | 6 | lld:pubmed |
| pubmed-article:14688405 | pubmed:volume | 101 | lld:pubmed |
| pubmed-article:14688405 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:14688405 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:14688405 | pubmed:pagination | 257-9 | lld:pubmed |
| pubmed-article:14688405 | pubmed:dateRevised | 2009-11-18 | lld:pubmed |
| pubmed-article:14688405 | pubmed:meshHeading | pubmed-meshheading:14688405... | lld:pubmed |
| pubmed-article:14688405 | pubmed:meshHeading | pubmed-meshheading:14688405... | lld:pubmed |
| pubmed-article:14688405 | pubmed:meshHeading | pubmed-meshheading:14688405... | lld:pubmed |
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| pubmed-article:14688405 | pubmed:meshHeading | pubmed-meshheading:14688405... | lld:pubmed |
| pubmed-article:14688405 | pubmed:meshHeading | pubmed-meshheading:14688405... | lld:pubmed |
| pubmed-article:14688405 | pubmed:meshHeading | pubmed-meshheading:14688405... | lld:pubmed |
| pubmed-article:14688405 | pubmed:meshHeading | pubmed-meshheading:14688405... | lld:pubmed |
| pubmed-article:14688405 | pubmed:meshHeading | pubmed-meshheading:14688405... | lld:pubmed |
| pubmed-article:14688405 | pubmed:year | 2004 | lld:pubmed |
| pubmed-article:14688405 | pubmed:articleTitle | Hybridoma-free generation of monoclonal antibodies. | lld:pubmed |
| pubmed-article:14688405 | pubmed:affiliation | University of Texas MD Anderson Cancer Center, Houston, TX 77030, USA. rpasqual@mdanderson.org | lld:pubmed |
| pubmed-article:14688405 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:14688405 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| pubmed-article:14688405 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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