|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
1
|
|
pubmed:dateCreated |
2004-1-7
|
|
pubmed:abstractText |
Production of monoclonal antibodies requires immortalization of splenocytes by somatic fusion to a myeloma cell line partner (hybridomas). Although hybridomas can be immortal, they may depend on a feeder cell layer and may be genetically unstable. Since the inception of hybridoma technology, efforts to improve efficiency and stability of monoclonal antibody-producing cell lines have not brought about substantial progress. Moreover, suitable human multiple myeloma-derived cell lines for the production of human antibodies have been very difficult to develop. Here we report a strategy that greatly simplifies the generation of antibodies and eliminates the need for hybridomas. We show that splenocytes derived from transgenic mice harboring a mutant temperature-sensitive simian virus 40 large tumor antigen under the control of a mouse major histocompatibility promoter are conditionally immortal at permissive temperatures and produce monoclonal antibodies. This simple approach may become a method of choice for generation and production of both polyclonal and monoclonal antibodies with advantages in high-throughput discovery and antibody-based immunotherapy.
|
|
pubmed:grant |
|
|
pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/14688405-11017048,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14688405-11172031,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14688405-1172191,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14688405-12782605,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14688405-1711218,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14688405-1987490,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14688405-6247241,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14688405-7678459,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14688405-8011287,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14688405-8159246,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14688405-8390670,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14688405-9020839,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14688405-9263031
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Jan
|
|
pubmed:issn |
0027-8424
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:day |
6
|
|
pubmed:volume |
101
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
257-9
|
|
pubmed:dateRevised |
2009-11-18
|
|
pubmed:meshHeading |
pubmed-meshheading:14688405-Animals,
pubmed-meshheading:14688405-Antibodies, Monoclonal,
pubmed-meshheading:14688405-Antigens, Polyomavirus Transforming,
pubmed-meshheading:14688405-Clone Cells,
pubmed-meshheading:14688405-H-2 Antigens,
pubmed-meshheading:14688405-Hybridomas,
pubmed-meshheading:14688405-Mice,
pubmed-meshheading:14688405-Mice, Transgenic,
pubmed-meshheading:14688405-Mutation,
pubmed-meshheading:14688405-Spleen,
pubmed-meshheading:14688405-Temperature
|
|
pubmed:year |
2004
|
|
pubmed:articleTitle |
Hybridoma-free generation of monoclonal antibodies.
|
|
pubmed:affiliation |
University of Texas MD Anderson Cancer Center, Houston, TX 77030, USA. rpasqual@mdanderson.org
|
|
pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
|
