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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
2003-12-22
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pubmed:abstractText |
IL-23 is a heterodimeric cytokine consisting of p19 and the p40 subunit of IL-12. IL-23 has been shown to possess IL-12-like biological activities, but is different in its capacity to stimulate memory T cells in vitro. In this study, we investigated whether IL-23 could influence envelope protein 2 (E2)-specific cell-mediated immunity induced by immunization of hepatitis C virus E2 DNA. We found that IL-23 induced long-lasting Th1 and CTL immune responses to E2, which are much stronger than IL-12-mediated immune responses. Interestingly, IL-23N220L, an N-glycosylation mutant showing reduced expression of excess p40 without changing the level of IL-23, exhibited a higher ratio of IFN-gamma- to IL-4-producing CD4(+) T cell frequency than did wild-type IL-23, suggesting a negative regulatory effect of p40 on Th1-prone immune response induced by IL-23. These data suggest that IL-23, particularly IL-23N220L, would be an effective adjuvant of DNA vaccine for the induction of durable Ag-specific T cell immunity.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
AIM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Adjuvants, Immunologic,
http://linkedlifedata.com/resource/pubmed/chemical/IL23A protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Il23a protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-12,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-12 Subunit p40,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-23,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-23 Subunit p19,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukins,
http://linkedlifedata.com/resource/pubmed/chemical/Protein Subunits,
http://linkedlifedata.com/resource/pubmed/chemical/Vaccines, DNA,
http://linkedlifedata.com/resource/pubmed/chemical/Viral Envelope Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/glycoprotein E2, Hepatitis C virus
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pubmed:status |
MEDLINE
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pubmed:month |
Jan
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pubmed:issn |
0022-1767
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
1
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pubmed:volume |
172
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
525-31
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:14688363-Adjuvants, Immunologic,
pubmed-meshheading:14688363-Amino Acid Sequence,
pubmed-meshheading:14688363-Animals,
pubmed-meshheading:14688363-Cytotoxicity, Immunologic,
pubmed-meshheading:14688363-Female,
pubmed-meshheading:14688363-Genetic Vectors,
pubmed-meshheading:14688363-Hepacivirus,
pubmed-meshheading:14688363-Humans,
pubmed-meshheading:14688363-Immunity, Cellular,
pubmed-meshheading:14688363-Injections, Intramuscular,
pubmed-meshheading:14688363-Interleukin-12,
pubmed-meshheading:14688363-Interleukin-12 Subunit p40,
pubmed-meshheading:14688363-Interleukin-23,
pubmed-meshheading:14688363-Interleukin-23 Subunit p19,
pubmed-meshheading:14688363-Interleukins,
pubmed-meshheading:14688363-Mice,
pubmed-meshheading:14688363-Mice, Inbred BALB C,
pubmed-meshheading:14688363-Molecular Sequence Data,
pubmed-meshheading:14688363-Protein Subunits,
pubmed-meshheading:14688363-T-Lymphocytes, Cytotoxic,
pubmed-meshheading:14688363-Th1 Cells,
pubmed-meshheading:14688363-Vaccines, DNA,
pubmed-meshheading:14688363-Viral Envelope Proteins
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pubmed:year |
2004
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pubmed:articleTitle |
IL-23 induces stronger sustained CTL and Th1 immune responses than IL-12 in hepatitis C virus envelope protein 2 DNA immunization.
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pubmed:affiliation |
National Research Laboratory of DNA Medicine, Division of Molecular and Life Sciences, Pohang University of Science and Technology, San 31 Hyoja-dong, Nam-gu, Pohang 790-784, Republic of Korea.
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pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, Non-U.S. Gov't
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