Source:http://linkedlifedata.com/resource/pubmed/id/14687726
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
2-3
|
| pubmed:dateCreated |
2003-12-22
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| pubmed:abstractText |
B lymphocytes express phospholipase C-gamma(1) (PLC-gamma(1)) and phospholipase C-gamma(2) (PLC-gamma(2)) isozymes. However, the relative importance of these two isozymes in B cell signaling is not known. We report here the identification and analysis of a B cell line deficient in PLC-gamma(1). Mature splenic B lymphocytes and a panel of cell lines representing pre-B, immature and mature B cell stages expressed phospholipase C-gamma (PLC-gamma), but not the beta or delta isoforms of phospholipase C (PLC). While all the tested B cell lines and primary splenic B cells expressed PLC-gamma(1) and PLC-gamma(2) isozymes, the L1.2 B cell line exclusively expressed PLC-gamma(2), but not PLC-gamma(1) isozyme. The PLC-gamma(1) deficient L1.2 B cells expressed levels of surface IgM comparable to that of PLC-gamma(1) positive 70Z/3 B cell line. However, stimulation through the antigen receptor induced Ca(++) response in 70Z/3, but not L1.2 B cells, suggesting a requirement for PLC-gamma(1) in antigen receptor induced Ca(++) signaling in these cells. The possible use for L1.2 cells in testing the regulation of PLC-gamma(1) and PLC-gamma(2) dependent calcium mobilization in B lymphocytes is discussed.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical | |
| pubmed:status |
MEDLINE
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| pubmed:month |
Dec
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| pubmed:issn |
0165-2478
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| pubmed:author | |
| pubmed:issnType |
Print
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| pubmed:day |
15
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| pubmed:volume |
90
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| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
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| pubmed:pagination |
203-7
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| pubmed:dateRevised |
2007-11-15
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| pubmed:meshHeading |
pubmed-meshheading:14687726-Animals,
pubmed-meshheading:14687726-B-Lymphocytes,
pubmed-meshheading:14687726-Calcium Signaling,
pubmed-meshheading:14687726-Cell Line, Tumor,
pubmed-meshheading:14687726-Immunoglobulin M,
pubmed-meshheading:14687726-Mice,
pubmed-meshheading:14687726-Phospholipase C gamma,
pubmed-meshheading:14687726-Receptors, Antigen, B-Cell,
pubmed-meshheading:14687726-Time Factors,
pubmed-meshheading:14687726-Type C Phospholipases
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| pubmed:year |
2003
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| pubmed:articleTitle |
Impaired antigen receptor induced calcium mobilization in a phospholipase C-gamma1 deficient B cell line.
|
| pubmed:affiliation |
Center for Molecular and Human Genetics, The Department of Pediatrics, Columbus Children's Research Institute, The Ohio State University Columbus, OH 43205, USA. rmuthusa@chi.osu.edu
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| pubmed:publicationType |
Journal Article
|