Source:http://linkedlifedata.com/resource/pubmed/id/14679398
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
5
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| pubmed:dateCreated |
2003-12-17
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| pubmed:abstractText |
Controversy exists over whether the predominant cell death of hepatocytes is due to apoptosis or necrosis after ischemia/reperfusion injury. In this study we investigated the predominant cell death of hepatocytes after cold ischemia/reperfusion injury using the Annexin V-based assay, and evaluated the anti-apoptotic effect of ascorbic acid 2-glucoside (AA-2G) added to the University of Wisconsin solution (UW solution) in rat liver transplantation. The retrieved liver was preserved in 4 UW solution for 24 h, and then transplanted orthotopically to the syngeneic Wistar recipient. The animals were divided into 2 groups, a control group (n=10), in which liver grafts were preserved in UW solution (4), and an AA-2G group (n=10), in which liver grafts were preserved in UW solution (4) with AA-2G (100 ug/ml). The serum AST level 4 h after reperfusion in the control group was significantly suppressed in the AA-2G group, and the bile production of the liver graft in the AA-2G group was well recovered. The mean survival time in the AA-2G group was significantly improved compared with that in the control group. Annexin-V and Propidium iodide staining 4 h after reperfusion showed a significantly higher percentage of viable hepatocytes in the AA-2G group compared with the control group (93.4 +/- 2.0 vs. 80.3 +- 2.1%, P<0.05). In the control group, the main cell death of hepatocytes was apoptosis (early apoptosis: 10.0 +- 4.7%, late apoptosis: 6.4 +/- 1.7%). The addition of AA-2G to the UW solution significantly inhibited both early and late apoptotic cell death 4 h after reperfusion (early apoptosis: 0.98 +/- 0.88%, late apoptosis: 2.2 +/- 1.1%). The expression of caspase 9 in the immunostaining of the liver graft was suppressed in the AA-2G group compared with in the control group. Our study using the Annexin V-based assay provided evidence that the predominant cell death of hepatocytes was apoptosis after 24 h cold ischemia/reperfusion injury in rat liver transplantation. The addition of AA-2G to the UW solution attenuated 24 h cold ischemia/reperfusion injury by inhibiting the apoptosis of hepatocytes.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Annexin A5,
http://linkedlifedata.com/resource/pubmed/chemical/Ascorbic Acid,
http://linkedlifedata.com/resource/pubmed/chemical/Aspartate Aminotransferases,
http://linkedlifedata.com/resource/pubmed/chemical/Casp9 protein, rat,
http://linkedlifedata.com/resource/pubmed/chemical/Caspase 9,
http://linkedlifedata.com/resource/pubmed/chemical/Caspases,
http://linkedlifedata.com/resource/pubmed/chemical/ascorbic acid 2-O-glucoside
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| pubmed:status |
MEDLINE
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| pubmed:month |
Oct
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| pubmed:issn |
0386-300X
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| pubmed:author |
pubmed-author:JinZaishunZ,
pubmed-author:LeeL FLF,
pubmed-author:MatsukawaHiroyoshiH,
pubmed-author:MatsuokaJunjiJ,
pubmed-author:MitsuokaNaoshiN,
pubmed-author:SadamoriHiroshiH,
pubmed-author:SunDong-ShengDS,
pubmed-author:TanakaNoriakiN,
pubmed-author:YagiTakahitoT,
pubmed-author:YamamotoItaruI,
pubmed-author:YamamuraMasaoM
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| pubmed:issnType |
Print
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| pubmed:volume |
57
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
209-16
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| pubmed:dateRevised |
2006-11-15
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| pubmed:meshHeading |
pubmed-meshheading:14679398-Animals,
pubmed-meshheading:14679398-Annexin A5,
pubmed-meshheading:14679398-Apoptosis,
pubmed-meshheading:14679398-Ascorbic Acid,
pubmed-meshheading:14679398-Aspartate Aminotransferases,
pubmed-meshheading:14679398-Bile,
pubmed-meshheading:14679398-Caspase 9,
pubmed-meshheading:14679398-Caspases,
pubmed-meshheading:14679398-Cryopreservation,
pubmed-meshheading:14679398-Flow Cytometry,
pubmed-meshheading:14679398-Hepatocytes,
pubmed-meshheading:14679398-Immunohistochemistry,
pubmed-meshheading:14679398-Liver,
pubmed-meshheading:14679398-Liver Circulation,
pubmed-meshheading:14679398-Liver Transplantation,
pubmed-meshheading:14679398-Male,
pubmed-meshheading:14679398-Rats,
pubmed-meshheading:14679398-Rats, Wistar,
pubmed-meshheading:14679398-Reperfusion Injury,
pubmed-meshheading:14679398-Staining and Labeling,
pubmed-meshheading:14679398-Survival Analysis
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| pubmed:year |
2003
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| pubmed:articleTitle |
Annexin V assay-proven anti-apoptotic effect of ascorbic acid 2-glucoside after cold ischemia/reperfusion injury in rat liver transplantation.
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| pubmed:affiliation |
Department of Gastroenterological Surgery, Transplant, and Surgical Oncology, Okayama University Graduate School of Medicine and Dentistry, Okayama 700-8558, Japan.
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| pubmed:publicationType |
Journal Article
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