Linked Life Data

14671010

Source:http://linkedlifedata.com/resource/pubmed/id/14671010

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2004-1-19
pubmed:abstractText
To identify genetic loci involved in the regulation of organ-specific enzyme activities, a specific histochemical staining protocol was used in combination with quantitative trait locus (QTL) analysis. Using phosphoglucomutase (PGM) as an example, it is shown that enzyme activity can specifically, and with high resolution, be visualized in non-sectioned seedlings of Arabidopsis. The intensities of staining were converted to quantitative data and used as trait for QTL analysis using Landsberg erecta x Cape Verde Islands recombinant inbred lines. Independently, PGM activities were quantified in whole-seedling extracts, and these data were also used for QTL analysis. On the basis of extract data, six significant (P < 0.05) loci affecting PGM activity were found. From the histochemical data, one or more specific QTLs were found for each organ analyzed (cotyledons, shoot apex, hypocotyl, root, root neck, root tip, and root hairs). Loci detected for PGM activity in extracts colocated with loci for histochemical staining. QTLs were found coinciding with positions of (putative) PGM genes but also at other positions, the latter ones supposedly pointing toward regulatory genes. Some of this type of loci were also organ specific. It is concluded that QTL analysis based on histochemical data is feasible and may reveal organ-specific loci involved in the regulation of metabolic pathways.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/14671010-10759515, http://linkedlifedata.com/resource/pubmed/commentcorrection/14671010-10954083, http://linkedlifedata.com/resource/pubmed/commentcorrection/14671010-11130711, http://linkedlifedata.com/resource/pubmed/commentcorrection/14671010-11743102, http://linkedlifedata.com/resource/pubmed/commentcorrection/14671010-11807140, http://linkedlifedata.com/resource/pubmed/commentcorrection/14671010-11810256, http://linkedlifedata.com/resource/pubmed/commentcorrection/14671010-11901129, http://linkedlifedata.com/resource/pubmed/commentcorrection/14671010-12376659, http://linkedlifedata.com/resource/pubmed/commentcorrection/14671010-12529542, http://linkedlifedata.com/resource/pubmed/commentcorrection/14671010-16664354, http://linkedlifedata.com/resource/pubmed/commentcorrection/14671010-9611188, http://linkedlifedata.com/resource/pubmed/commentcorrection/14671010-9628021, http://linkedlifedata.com/resource/pubmed/commentcorrection/14671010-9715535, http://linkedlifedata.com/resource/pubmed/commentcorrection/14671010-9808723
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
0032-0889
pubmed:author
pubmed:issnType
Print
pubmed:volume
134
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
237-45
pubmed:dateRevised
2010-9-21
pubmed:meshHeading
pubmed:year
2004
pubmed:articleTitle
Histochemical analysis reveals organ-specific quantitative trait loci for enzyme activities in Arabidopsis.
pubmed:affiliation
Laboratory of Plant Physiology, Graduate School Experimental Plant Sciences, Wageningen University, Arboretumlaan 4, 6703 BD Wageningen, The Netherlands.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't