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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
4
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pubmed:dateCreated |
2003-12-3
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pubmed:abstractText |
Scavenger receptor class B type I (SR-BI) is an HDL receptor that mediates selective HDL lipid uptake. Peroxisomes play an important role in lipid metabolism and peroxisomal targeting signal type 1 (PTS1)-containing proteins are translocated to peroxisomes by the peroxisomal targeting import receptor, Pex5p. We have previously identified a PTS1 motif in the intracellular domain of rat SR-BI. Here, we examine the possible interaction between Pex5p and SR-BI. Expression of a Flag-tagged intracellular domain of SR-BI resulted in translocation to the peroxisome as demonstrated by double labeling with anti-Flag IgG and anti-catalase IgG analyzed by confocal microscopy. Immunoprecipitation experiments with anti-SR-BI antibody showed that Pex5p co-precipitated with SR-BI. However, when an antibody against Pex5p was used for immunoprecipitation, only the 57kDa, non-glycosylated form, of SR-BI co-precipitated. We conclude that the PTS1 domain of SR-BI is functional and can mediate peroxisomal interaction via Pex5p, in vitro.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD36,
http://linkedlifedata.com/resource/pubmed/chemical/Lipoproteins, HDL,
http://linkedlifedata.com/resource/pubmed/chemical/Membrane Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Cytoplasmic and Nuclear,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Immunologic,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Lipoprotein,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Scavenger,
http://linkedlifedata.com/resource/pubmed/chemical/Scarb1 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Scarb1 protein, rat,
http://linkedlifedata.com/resource/pubmed/chemical/Scavenger Receptors, Class B,
http://linkedlifedata.com/resource/pubmed/chemical/peroxisome-targeting signal 1...
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pubmed:status |
MEDLINE
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pubmed:month |
Dec
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pubmed:issn |
0006-291X
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
26
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pubmed:volume |
312
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1325-34
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:14652019-Amino Acid Sequence,
pubmed-meshheading:14652019-Animals,
pubmed-meshheading:14652019-Antigens, CD36,
pubmed-meshheading:14652019-Binding Sites,
pubmed-meshheading:14652019-CHO Cells,
pubmed-meshheading:14652019-COS Cells,
pubmed-meshheading:14652019-Cercopithecus aethiops,
pubmed-meshheading:14652019-Cricetinae,
pubmed-meshheading:14652019-Cricetulus,
pubmed-meshheading:14652019-Lipoproteins, HDL,
pubmed-meshheading:14652019-Membrane Proteins,
pubmed-meshheading:14652019-Molecular Biology,
pubmed-meshheading:14652019-Peroxisomes,
pubmed-meshheading:14652019-Protein Binding,
pubmed-meshheading:14652019-Protein Structure, Tertiary,
pubmed-meshheading:14652019-Rats,
pubmed-meshheading:14652019-Receptors, Cytoplasmic and Nuclear,
pubmed-meshheading:14652019-Receptors, Immunologic,
pubmed-meshheading:14652019-Receptors, Lipoprotein,
pubmed-meshheading:14652019-Receptors, Scavenger,
pubmed-meshheading:14652019-Scavenger Receptors, Class B,
pubmed-meshheading:14652019-Sequence Homology, Amino Acid,
pubmed-meshheading:14652019-Structure-Activity Relationship
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pubmed:year |
2003
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pubmed:articleTitle |
Interaction of scavenger receptor class B type I with peroxisomal targeting receptor Pex5p.
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pubmed:affiliation |
Research Centre for Endocrinology and Metabolism (RCEM), Department of Internal Medicine, The Sahlgrenska Academy at Göteborg University, S-413 45, Göteborg, Sweden. Magnus.Johnson@medic.gu.se
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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