Source:http://linkedlifedata.com/resource/pubmed/id/14647990
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
4
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pubmed:dateCreated |
2004-4-16
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pubmed:abstractText |
Crude cell extracts of Pseudomonas putida F6 transformed 4-substituted fluoro-, chloro-, bromo- and iodo-phenol without the exogenous addition of cofactors. The rate of substrate consumption decreased with increasing substituent size (F>Cl>Br>I). Biotransformations resulted in greater than 95% utilisation of the halogenated substrate. Product accumulation was observed in incubations with 4-chloro, 4-bromo- and 4-iodo-phenol. These products were identified as the corresponding 4-substituted catechols. Transformation of 4-fluorophenol did not result in the accumulation of the corresponding catechol; however, manipulation of the reaction conditions by incorporation of ascorbic acid culminated in the formation of 4-fluorocatechol. Cell extracts of P. putida F6 also showed activity towards a 3-substituted phenol, namely 3-fluorophenol, resulting in the formation of a single product, 4-fluorocatechol.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/3-fluorophenol,
http://linkedlifedata.com/resource/pubmed/chemical/4-bromophenol,
http://linkedlifedata.com/resource/pubmed/chemical/4-chlorophenol,
http://linkedlifedata.com/resource/pubmed/chemical/4-fluorophenol,
http://linkedlifedata.com/resource/pubmed/chemical/4-iodophenol,
http://linkedlifedata.com/resource/pubmed/chemical/Catechols,
http://linkedlifedata.com/resource/pubmed/chemical/Chlorophenols,
http://linkedlifedata.com/resource/pubmed/chemical/Coenzymes,
http://linkedlifedata.com/resource/pubmed/chemical/Iodobenzenes,
http://linkedlifedata.com/resource/pubmed/chemical/Phenols
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
0175-7598
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pubmed:author | |
pubmed:copyrightInfo |
Copyright 2003 Springer-Verlag
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pubmed:issnType |
Print
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pubmed:volume |
64
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
486-92
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:14647990-Biotransformation,
pubmed-meshheading:14647990-Catechols,
pubmed-meshheading:14647990-Chlorophenols,
pubmed-meshheading:14647990-Coenzymes,
pubmed-meshheading:14647990-Iodobenzenes,
pubmed-meshheading:14647990-Phenols,
pubmed-meshheading:14647990-Pseudomonas putida,
pubmed-meshheading:14647990-Substrate Specificity
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pubmed:year |
2004
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pubmed:articleTitle |
Biotransformation of halophenols using crude cell extracts of Pseudomonas putida F6.
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pubmed:affiliation |
Department of Industrial Microbiology, University College Dublin, Belfield Dublin 4, Republic of Ireland.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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