Linked Life Data

14624533

Source:http://linkedlifedata.com/resource/pubmed/id/14624533

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
2003-11-18
pubmed:abstractText
The in vivo osteogenic potential of autogenous cultured bone/ceramic constructs in large animals or humans is unknown, and thus we performed a preliminary study of this issue prior to clinical application. All autogenous cultured-bone/ceramic constructs at 3 weeks after implantation in dogs showed obvious histological bone formation within the ceramic pores. In many pores, the HE staining of decalcified specimens revealed thick lamellar bone formation on the pore surface of ceramic. On the surface of bone tissue, numerous active cuboidal osteoblasts were evident. Biochemically, high alkaline phosphatase activity was detected in all dogs. Histological examination of the constructs at 8 weeks postimplantation showed lamellar bone formation with vascular system invasion into the pores, and regenerated hematopoietic bone marrow was often detected in association with the new bone in grafting of human cultured bone/ceramic constructs. Trilineage hematopoietic cells (i.e., granulocytic, erythroblastic, and megakaryocytic cells) were identified in the ceramic pores. Biochemically, high alkaline phosphatase activity and significant human osteocalcin content was detected in the constructs. Based on these findings, in the near future, this technique (grafting of patient-derived cultured bone/HA constructs) will be able to be applied to various bone reconstruction surgical treatments.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
1549-3296
pubmed:author
pubmed:copyrightInfo
Copyright 2003 Wiley Periodicals, Inc. J Biomed Mater Res 67A: 1437-1441, 2003
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
67
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1437-41
pubmed:dateRevised
2004-12-15
pubmed:meshHeading
pubmed:year
2003
pubmed:articleTitle
Bone regeneration by grafting of an autogenous cultured bone/ceramic construct.
pubmed:affiliation
Departments of Orthopaedic Surgery, Nara Medical University, Kashihara City, Nara 634-8522, Japan. tyoshi@naramed-u.ac.jp
pubmed:publicationType
Journal Article