Source:http://linkedlifedata.com/resource/pubmed/id/14583637
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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
4
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pubmed:dateCreated |
2003-10-29
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pubmed:abstractText |
A multiplex polymerase chain reaction (PCR) assay was developed for the identification of Salmonella enterica serovar Typhimurium. Three sets of primers were designed for detecting O4, H:i, and H:1,2 antigen genes from the antigen-specific genes rfbJ, fliC, and fljB, respectively. These were evaluated in a multiplex PCR assay by using DNAs from S. enterica serovar Typhimurium, 15 other Salmonella serovars, and 8 non-Salmonella enteric pathogens. Multiplex PCR proved to be capable of identifying S. enterica serovar Typhimurium specifically and differentiating it from other Salmonella serovars in addition to non-Salmonella enteric pathogens. Thus, this multiplex PCR assay can be practically applied to the identification of S. enterica serovar Typhimurium.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
1344-6304
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
56
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
151-5
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading | |
pubmed:year |
2003
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pubmed:articleTitle |
Multiplex polymerase chain reaction assay for selective detection of Salmonella enterica serovar typhimurium.
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pubmed:affiliation |
Department of Clinical Laboratory Science, College of Health Sciences, Korea University, Seoul 136-703, Korea.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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