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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
6
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pubmed:dateCreated |
2003-10-28
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pubmed:abstractText |
Normal human hepatocytes are an ideal source of liver-targeted cell therapies, such as hepatocyte transplantation and bioartificial livers, but availability of human donor livers for liver cell isolation is severely limited. To effectively utilize scarce donor organs for cell therapies, it is of extreme importance to establish an efficient isolation technique and an effective cold preservation solution for transportation of isolated cells. A lateral segment of the liver was surgically resected from pigs weighing 10 kg and a four-step collagenase and dispase digestion was conducted. Isolated hepatocytes were subjected to 8-h cold storage on ice. The following preservation solutions were tested: 1) University of Wisconsin (UW) solution, 2) UW with 100 microg/ml of ascorbic acid-2 glucoside (AA2G), 3) 100% fetal bovine serum (FBS), and 4) Dulbecco's modified Eagle's medium (DMEM) supplemented with 100% FBS. The mean viability of porcine hepatocytes was 95.5 +/- 2.5% when isolated in three independent experiments. Viability, plating efficiency, membrane stability, and ammonia metabolic capacity of cold-preserved hepatocytes were significantly better maintained by the use of UW solution. When AA2G (100 microg/ml) was combined with UW solution, such parameters were further improved. It was explained by inhibition of caspase-3 activation and retention of ATP at high levels of hepatocytes preserved with UW solution containing AA2G. The present work demonstrates that a combination of UW solution with AA2G (100 microg/ml) would be a useful cold preservation means for the development of cell therapies.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Adenosine,
http://linkedlifedata.com/resource/pubmed/chemical/Adenosine Triphosphate,
http://linkedlifedata.com/resource/pubmed/chemical/Allopurinol,
http://linkedlifedata.com/resource/pubmed/chemical/Ammonia,
http://linkedlifedata.com/resource/pubmed/chemical/Ascorbic Acid,
http://linkedlifedata.com/resource/pubmed/chemical/CASP3 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Caspase 3,
http://linkedlifedata.com/resource/pubmed/chemical/Caspases,
http://linkedlifedata.com/resource/pubmed/chemical/Cryoprotective Agents,
http://linkedlifedata.com/resource/pubmed/chemical/Glutathione,
http://linkedlifedata.com/resource/pubmed/chemical/Insulin,
http://linkedlifedata.com/resource/pubmed/chemical/Organ Preservation Solutions,
http://linkedlifedata.com/resource/pubmed/chemical/Raffinose,
http://linkedlifedata.com/resource/pubmed/chemical/University of...,
http://linkedlifedata.com/resource/pubmed/chemical/ascorbic acid 2-O-glucoside
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pubmed:status |
MEDLINE
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pubmed:issn |
0963-6897
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pubmed:author |
pubmed-author:ArataAkiraA,
pubmed-author:IkedaHideakiH,
pubmed-author:KobayashiNaoyaN,
pubmed-author:KodamaMakotoM,
pubmed-author:KosakaYoshikazuY,
pubmed-author:KuniedaTakemiT,
pubmed-author:KurabayashiYuzuruY,
pubmed-author:LobontiuAdrianA,
pubmed-author:MatsuokaJunjiJ,
pubmed-author:OhmotoKenjiK,
pubmed-author:OkitsuTeruT,
pubmed-author:OyamaToshieT,
pubmed-author:SakaguchiMasakiyoM,
pubmed-author:ShibataNorikuniN,
pubmed-author:TakesueMichihikoM,
pubmed-author:TanakaNoriakiN,
pubmed-author:TotsugawaToshinoriT,
pubmed-author:YamamotoItaruI,
pubmed-author:YamamotoShinichiroS
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pubmed:issnType |
Print
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pubmed:volume |
12
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
599-606
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pubmed:dateRevised |
2011-11-17
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pubmed:meshHeading |
pubmed-meshheading:14579928-Adenosine,
pubmed-meshheading:14579928-Adenosine Triphosphate,
pubmed-meshheading:14579928-Allopurinol,
pubmed-meshheading:14579928-Ammonia,
pubmed-meshheading:14579928-Animals,
pubmed-meshheading:14579928-Apoptosis,
pubmed-meshheading:14579928-Ascorbic Acid,
pubmed-meshheading:14579928-Caspase 3,
pubmed-meshheading:14579928-Caspases,
pubmed-meshheading:14579928-Cell Culture Techniques,
pubmed-meshheading:14579928-Cell Membrane,
pubmed-meshheading:14579928-Cell Separation,
pubmed-meshheading:14579928-Cell Survival,
pubmed-meshheading:14579928-Cell Transplantation,
pubmed-meshheading:14579928-Cells, Cultured,
pubmed-meshheading:14579928-Cryopreservation,
pubmed-meshheading:14579928-Cryoprotective Agents,
pubmed-meshheading:14579928-Glutathione,
pubmed-meshheading:14579928-Hepatocytes,
pubmed-meshheading:14579928-Insulin,
pubmed-meshheading:14579928-Liver Diseases,
pubmed-meshheading:14579928-Liver Transplantation,
pubmed-meshheading:14579928-Male,
pubmed-meshheading:14579928-Organ Preservation Solutions,
pubmed-meshheading:14579928-Raffinose,
pubmed-meshheading:14579928-Sus scrofa,
pubmed-meshheading:14579928-Transplantation, Heterologous
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pubmed:year |
2003
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pubmed:articleTitle |
Maintenance of cold-preserved porcine hepatocyte function with UW solution and ascorbic acid-2 glucoside.
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pubmed:affiliation |
Division of Gastroenterology, Department of Internal Medicine, Kawasaki Medical School, 577 Matsushima, Kurashiki 701-0192, Japan.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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