Linked Life Data

14574680

Source:http://linkedlifedata.com/resource/pubmed/id/14574680

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2003-10-23
pubmed:abstractText
Gamma-aminobutyric acid (GABA)ergic neurons in the central nervous system regulate the activity of other neurons and play a crucial role in information processing. To assist an advance in the research of GABAergic neurons, here we produced two lines of glutamic acid decarboxylase-green fluorescence protein (GAD67-GFP) knock-in mouse. The distribution pattern of GFP-positive somata was the same as that of the GAD67 in situ hybridization signal in the central nervous system. We encountered neither any apparent ectopic GFP expression in GAD67-negative cells nor any apparent lack of GFP expression in GAD67-positive neurons in the two GAD67-GFP knock-in mouse lines. The timing of GFP expression also paralleled that of GAD67 expression. Hence, we constructed a map of GFP distribution in the knock-in mouse brain. Moreover, we used the knock-in mice to investigate the colocalization of GFP with NeuN, calretinin (CR), parvalbumin (PV), and somatostatin (SS) in the frontal motor cortex. The proportion of GFP-positive cells among NeuN-positive cells (neocortical neurons) was approximately 19.5%. All the CR-, PV-, and SS-positive cells appeared positive for GFP. The CR-, PV, and SS-positive cells emitted GFP fluorescence at various intensities characteristics to them. The proportions of CR-, PV-, and SS-positive cells among GFP-positive cells were 13.9%, 40.1%, and 23.4%, respectively. Thus, the three subtypes of GABAergic neurons accounted for 77.4% of the GFP-positive cells. They accounted for 6.5% in layer I. In accord with unidentified GFP-positive cells, many medium-sized spherical somata emitting intense GFP fluorescence were observed in layer I.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0021-9967
pubmed:author
pubmed:copyrightInfo
Copyright 2003 Wiley-Liss, Inc.
pubmed:issnType
Print
pubmed:day
1
pubmed:volume
467
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
60-79
pubmed:dateRevised
2007-10-24
pubmed:meshHeading
pubmed-meshheading:14574680-Animals, pubmed-meshheading:14574680-Blotting, Western, pubmed-meshheading:14574680-Calcium-Binding Protein, Vitamin D-Dependent, pubmed-meshheading:14574680-Central Nervous System, pubmed-meshheading:14574680-Gene Expression, pubmed-meshheading:14574680-Glutamate Decarboxylase, pubmed-meshheading:14574680-Green Fluorescent Proteins, pubmed-meshheading:14574680-Immunohistochemistry, pubmed-meshheading:14574680-In Situ Hybridization, pubmed-meshheading:14574680-Isoenzymes, pubmed-meshheading:14574680-Luminescent Proteins, pubmed-meshheading:14574680-Mice, pubmed-meshheading:14574680-Mice, Neurologic Mutants, pubmed-meshheading:14574680-Motor Cortex, pubmed-meshheading:14574680-Neurons, pubmed-meshheading:14574680-Parvalbumins, pubmed-meshheading:14574680-Somatostatin, pubmed-meshheading:14574680-gamma-Aminobutyric Acid
pubmed:year
2003
pubmed:articleTitle
Green fluorescent protein expression and colocalization with calretinin, parvalbumin, and somatostatin in the GAD67-GFP knock-in mouse.
pubmed:affiliation
Department of Morphological Brain Science, Graduate School of Medicine, Kyoto University, Kyoto 606-8501, Japan. tamamaki@mbs.med.kyoto-u.ac.jp
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't