Linked Life Data

14573522

Source:http://linkedlifedata.com/resource/pubmed/id/14573522

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
24
pubmed:dateCreated
2003-11-4
pubmed:abstractText
Helix-loop-helix transcription factors of the Ebf/Olf1 family have previously been implicated in the control of neurogenesis in the central nervous system in both Xenopus laevis and the mouse, but their precise roles have remained unclear. We have characterised two family members in the chick, and have performed a functional analysis by gain- and loss-of-function experiments. This study revealed several specific roles for Ebf genes in the spinal cord and hindbrain regions of higher vertebrates, and enabled their precise positioning along the neurogenic cascade. During neurogenesis, cell cycle exit appears to be tightly coupled to migration to the mantle layer and to neuronal differentiation. We show that antagonizing Ebf gene activity allows the uncoupling of these processes. Ebf gene function is necessary to initiate neuronal differentiation and migration toward the mantle layer in neuroepithelial progenitors, but it is not required for cell cycle exit. Ebf genes therefore appear to be master controllers of neuronal differentiation and migration, coupling them to cell cycle exit and earlier steps of neurogenesis. Mutual activation between proneural and Ebf genes suggests that besides their involvement in the engagement of differentiation, Ebf genes may also participate in the stabilisation of the committed state. Finally, gain-of-function data raise the possibility that, in addition to these general roles, Ebf genes may be involved in neuronal subtype specification in particular regions of the CNS.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0950-1991
pubmed:author
pubmed:issnType
Print
pubmed:volume
130
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
6013-25
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:14573522-Animals, pubmed-meshheading:14573522-Avian Proteins, pubmed-meshheading:14573522-Basic Helix-Loop-Helix Transcription Factors, pubmed-meshheading:14573522-Cell Cycle, pubmed-meshheading:14573522-Cell Differentiation, pubmed-meshheading:14573522-Cell Lineage, pubmed-meshheading:14573522-Cell Movement, pubmed-meshheading:14573522-Chick Embryo, pubmed-meshheading:14573522-DNA-Binding Proteins, pubmed-meshheading:14573522-Gene Expression Regulation, Developmental, pubmed-meshheading:14573522-In Situ Hybridization, pubmed-meshheading:14573522-Nerve Tissue Proteins, pubmed-meshheading:14573522-Neurons, pubmed-meshheading:14573522-Neuropeptides, pubmed-meshheading:14573522-Trans-Activators, pubmed-meshheading:14573522-Transcription Factors, pubmed-meshheading:14573522-Xenopus Proteins
pubmed:year
2003
pubmed:articleTitle
Ebf gene function is required for coupling neuronal differentiation and cell cycle exit.
pubmed:affiliation
Unité 368 de l'Institut National de la Santé et de la Recherche Médicale, Ecole Normale Supérieure, 46 rue d'Ulm, 75230 Paris 05, France.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't