Linked Life Data

14532098

Source:http://linkedlifedata.com/resource/pubmed/id/14532098

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
10
pubmed:dateCreated
2003-10-8
pubmed:abstractText
Teschoviruses specifically infect pigs and are shed in pig feces. Hence, their presence in water should indicate contamination with pig fecal residues. To assess this hypothesis, we have developed a real-time reverse transcriptase PCR (RT-PCR) method that allows the quantitative detection of pig teschovirus (PTV) RNA. The method is able to detect 92 fg of PTV RNA per ml of sample. Using this method, we have detected the presence of PTV RNA in water and fecal samples from all pig farms examined (n = 5). Feces from other animal species (cattle, sheep, and goats) were negative in this test. To compare the PTV RNA detection method with conventional chemical determinations currently in use for evaluation of water contamination, we analyzed water samples collected downstream from a pig slurry spillage site. We have found a positive correlation within both types of determinations. The sensitivity of the PTV detection assay was similar to that achieved by unspecific organic matter determination and superior to all other conventional chemical analyses performed. Furthermore, the new method is highly specific, revealing the porcine origin of the contamination, a feature that is lacking in currently available methods for the assessment of water contamination.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/14532098-10100674, http://linkedlifedata.com/resource/pubmed/commentcorrection/14532098-10466788, http://linkedlifedata.com/resource/pubmed/commentcorrection/14532098-10542031, http://linkedlifedata.com/resource/pubmed/commentcorrection/14532098-11160660, http://linkedlifedata.com/resource/pubmed/commentcorrection/14532098-11161281, http://linkedlifedata.com/resource/pubmed/commentcorrection/14532098-11444000, http://linkedlifedata.com/resource/pubmed/commentcorrection/14532098-11992011, http://linkedlifedata.com/resource/pubmed/commentcorrection/14532098-12089028, http://linkedlifedata.com/resource/pubmed/commentcorrection/14532098-12462576, http://linkedlifedata.com/resource/pubmed/commentcorrection/14532098-9302419, http://linkedlifedata.com/resource/pubmed/commentcorrection/14532098-9814905
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0099-2240
pubmed:author
pubmed:issnType
Print
pubmed:volume
69
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
6311-5
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
2003
pubmed:articleTitle
Teschoviruses as indicators of porcine fecal contamination of surface water.
pubmed:affiliation
Departamento de Biotecnología, Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria, 28040 Madrid, Spain.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't, Evaluation Studies