Linked Life Data

14517218

Source:http://linkedlifedata.com/resource/pubmed/id/14517218

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
50
pubmed:dateCreated
2003-12-8
pubmed:abstractText
Several short, highly cationic peptides are able to enter the cytoplasm and nucleus of cells from the extracellular medium. The mechanism of entry is unknown. A number of fluorescence-based studies suggested that these molecules cross the plasma membrane by an energy-independent process, directly gaining access to the cytoplasm. Recent reports have questioned this conclusion, attributing the prior observations to artifacts resulting from fixation procedures used to prepare cells for fluorescence microscopy. These studies analyzed live cells and showed that the peptides entered through endocytosis and accumulated in endocytic vesicles, without necessarily entering the cytoplasm. To resolve this controversy and to extend the analyses to non-natural beta-peptide sequences, we studied the cytoplasmic and nuclear delivery of a fluorescein-labeled 9-residue sequence derived from the human immunodeficiency virus transactivator of transcription (TAT) peptide, TAT-(47-57), as well as a similarly labeled 12-residue beta-peptide, beta-(VRR)4, in live cells. Using fluorescence confocal microscopy, we show that when added to cells, both peptides are found in endocytic vesicles containing the transferrin receptor as well as in the cytoplasm and nucleus (TAT-(47-57)) or nucleolus (beta-(VRR)4). The cells were verified to be intact through all experimental procedures by demonstrating their ability to exclude propidium iodide. Endocytic entry of the peptides was blocked by the energy poisons sodium azide and 2-deoxyglucose, whereas staining of the nucleus (nucleolus), but not endocytic vesicles, was abrogated by treating the cells with ammonium chloride. Our observations are consistent with the proposal that TAT-(47-57) and beta-(VRR)4 enter cells by endocytosis and then exit an endosomal compartment to enter the cytoplasm by means of a mechanism requiring endosome acidification.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0021-9258
pubmed:author
pubmed:issnType
Print
pubmed:day
12
pubmed:volume
278
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
50188-94
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed-meshheading:14517218-Active Transport, Cell Nucleus, pubmed-meshheading:14517218-Adenosine Triphosphate, pubmed-meshheading:14517218-Ammonium Chloride, pubmed-meshheading:14517218-Cations, pubmed-meshheading:14517218-Cell Membrane, pubmed-meshheading:14517218-Cell Nucleus, pubmed-meshheading:14517218-Cytoplasm, pubmed-meshheading:14517218-Endocytosis, pubmed-meshheading:14517218-Endosomes, pubmed-meshheading:14517218-Gene Products, tat, pubmed-meshheading:14517218-HeLa Cells, pubmed-meshheading:14517218-Humans, pubmed-meshheading:14517218-Microscopy, Confocal, pubmed-meshheading:14517218-Microscopy, Fluorescence, pubmed-meshheading:14517218-Models, Biological, pubmed-meshheading:14517218-Models, Chemical, pubmed-meshheading:14517218-Peptides, pubmed-meshheading:14517218-Protein Transport, pubmed-meshheading:14517218-Receptors, Transferrin
pubmed:year
2003
pubmed:articleTitle
Cytoplasmic and nuclear delivery of a TAT-derived peptide and a beta-peptide after endocytic uptake into HeLa cells.
pubmed:affiliation
Department of Chemistry, University of Wisconsin, Madison, Wisconsin 53706, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.